Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/10931
Título : Citric acid production and citrate synthase genes in distinct strains of Aspergillus niger
Autor: Costa, Livia Martinez Abreu Soares
Chalfoun, Souza Sara Maria
Abreu, Priscilla Silva de
Bastos, Sabrina Carvalho
Nascimento, Marilza Neves do
Mallet, Aline Cristina Teixeira
Palavras-chave: Biotechnology
Enzyme
Strains
Publicador: African Journals Online
Data da publicação: 2015
Referência: COSTA, L. M. A. S. et al. Citric acid production and citrate synthase genes in distinct strains of Aspergillus niger. African Journal of Biotechnology, Nairobi, v. 13, n. 22, p. 2220-2226, 2015.
Abstract: Citric acid is an important organic acid, multifunctional with a wide array of uses. The objectives of this study were the isolation and selection strains of the genus Aspergillus, investigating the solubilization of phosphate of these isolates, verifying the expression rate of genes involved in the identification of isolates, and efficiency of citrate synthase in the citric acid production. To verify the mechanisms of the microorganisms in solubilizing phosphate into the medium in the citric acid production, Araxá rock phosphate was utilized. Further, citric acid was evaluated in two culture media, namely BD and SA. The amplification by the polymerase chain reaction was done by using primer ITS region and citrate synthase (P1/P2). The isolates of Aspergillus niger were efficient to solubilize phosphate. The isolate A. niger 00118 stood out in the solubilization with an increase as high as five times the amount of soluble phosphorus when compared with the control treatment. The results of citric acid production highlighted better influence conditions of the culture medium Sabouraud (SA) on its production. The P1/P2 primer was sensitive in distinguishing the isolates A. niger 00116, A. niger 00104, A. niger 00098 and A. niger 00118 in the analysis of citrate synthase enzyme genes.
URI: http://www.ajol.info/index.php/ajb/article/view/120154
http://repositorio.ufla.br/jspui/handle/1/10931
Idioma: en_US
Aparece nas coleções:DCA - Artigos publicados em periódicos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.