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metadata.artigo.dc.title: Immune response of bovines stimulated by synthetic vaccine SBm74621 against Rhipicephalus (Boophilus) microplus
metadata.artigo.dc.creator: Patarroyo, Joaquin Hernan
Vargas, Marlene isabel
Gonzáles, C. Z.
Guzman, F.
Martins Filho, Olindo Assis
Afonso, Luís Carlos Crocco
Valente, Fabricio Luciani
Peconick, Ana Paula
Marciano, A. P.
Patarroyo-Vargas, Angélica Maria
Sossai, Sidimar
metadata.artigo.dc.subject: Control ticks
Rhipicephalus (Boophilus) microplus
Synthetic vaccine
Immune response
Controle de carrapatos
Vacina sintética
Resposta imune
metadata.artigo.dc.publisher: Elsevier Science Publishers Dec-2009
metadata.artigo.dc.identifier.citation: PATARROYO, J. H. et al. Immune response of bovines stimulated by synthetic vaccine SBm74621 against Rhipicephalus (Boophilus) microplus. Veterinary Parasitology, Amsterdam, v. 166, n. 3/4, p. 333-339, Dec. 2009.
metadata.artigo.dc.description.abstract: Ten-month-old calves Bos taurus taurus were immunized with three doses of SBm74621 with saponin as an adjuvant at 30-day intervals and were evaluated for IgG isotypes, phenotype circulating lymphocytes and changes in the lymph nodes (LN). SBm74621 stimulated the production of predominantly IgG1-isotype IgG antibodies. The lymph nodes exhibited activation at the seventh day after the first immunization, with areas of paracortical and interfollicular hyperplasia and the early formation of germinal centers (GC). Fifteen days after the first immunization, the GC exhibited compartmentalization of cellular populations, a light zone (LZ), a dark zone (DZ) and a mantle. At the same time, hyperplasia of the medullary cords was observed with cells associating with DC cells. Seven days after the first immunization, apoptosis in the DZ and in the paracortical region became evident. By day 15, there was an increase in the medullary cords, which became more numerous at days 35 and 42. PAP-positive cells were found in the paracortical region, medullary cords and GC 7 days after the first immunization. At day 35, there were further strongly PAP-positive cells in the medullary cords. By comparison, none of these changes were observed in the lymph nodes of control groups at any of the days analyzed. The number of CD21+ lymphocytes increased in the immunized groups after the first inoculation, with a maximum number observed at 15 and 10 days after the first and third immunizations, respectively. Compared to pre-immunization counts, the percentage of WC1+ gd T-lymphocytes displayed more variation, increasing 5 days after the second immunization but decreasing over the following days. According to the results, the synthetic anti Rhipicephalus microplus vaccine elicits a complete immune response being T-dependant.
metadata.artigo.dc.language: pt_BR
Appears in Collections:DMV - Artigos publicados em periódicos

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