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|Title: ||In vivo and in vitro study of the effects of entomopathogenic bacteria and their filtrates on Meloidogyne incognita|
|???metadata.dc.creator???: ||Andaló, Vanessa|
Campos, Vicente Paulo
Moino Júnior, Alcides
Rocha, Fernando S.
|Keywords: ||Nematóides – Patogênese|
Agentes no controle biológico de pragas
Nematoda – Pathogenesis
Biological pest control agents
|Publisher: ||International Research Journals|
|Issue Date: ||2012|
|Citation: ||ANDALÓ, V. et al. In vivo and in vitro study of the effects of entomopathogenic bacteria and their filtrates on Meloidogyne incógnita. International Research Journal of Microbiology, [S. l.], v. 3, n. 1, p. 5-9, Jan. 2012.|
|Abstract: ||Symbiotic bacteria associated with entomopathogenic nematodes produce compounds with
antibacterial and antifungal activity. In the present study, Xenorhabdus spp. and Photorhabdus spp.
were cultivated in culture medium (in vitro) and in Galleria mellonella (L.) infected with
entomopathogenic nematodes (in vivo), and the effect of their filtrates on the mortality of Meloidogyne
incognita second stage juveniles (J2s) was investigated. The bacteria filtrates obtained by media
culture and hemolymph were associated with different species of entomopathogenic nematodes and
placed in direct contact with J2s of M. incognita. The mortality of the juveniles was estimated after 24 h.
After dilution of the hemolymph from infected G. mellonella, the filtrates obtained from the in vivo
culture and in vitro culture produced an 80% and 20% mortality rate, respectively. Thus, the quality of
substances with nematicidal activity was affected by the technique used to cultivate the bacteria,
reflecting the difference between cultivation in vivo and in vitro and the importance of the insect host.|
|Appears in Collections:||DEN - Artigos publicados em periódicos|
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