Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/11744
Título : Gene expression associated with the biosynthetic pathway of abscisic acid, gibberellin and ethylene during lettuce seed development
Autor: Clemente, Aline da Consolação Sampaio
Guimarães, Renato Mendes
Martins, Denize Carvalho
Lima, André Almeida
Gomes, Luiz Antonio Augusto
Baldoni, Alexana
Palavras-chave: Phytohormones
Seed maturation
Real time polymerase chain reaction
Publicador: African Journals Online
Data da publicação: 8-Set-2016
Referência: CLEMENTE, A. da C. S. et al. Gene expression associated with the biosynthetic pathway of abscisic acid, gibberellin and ethylene during lettuce seed development. African Journal of Biotechnology, Nairobi, v. 12, n. 23, p. 3676-3684, June 2013.
Abstract: Seed development may be divided up into embryonic morphogenesis and seed maturation, while diverse genes were connected and disconnected in order to bring about such processes. Features such as desiccation tolerance, dormancy and germination capacity are acquired throughout development and regulated by the presence and interaction of phytohormones such as abscisic acid (ABA), gibberellin (GA) and ethylene. The purpose of this study was to analyze the expression of genes connected with ABA, GA and ethylene biosynthesis during the development of lettuce seeds. For that reason, expression of the LsNCED, LsGAox and ACO-B genes was evaluated by means of the real time polymerase chain reaction (PCR) technique in seeds from seven lettuce cultivars harvested at the maturation stages of 5, 10, 15 and 20 days after anthesis. It may be concluded that expression of the LsNCED gene was related to the imposition of dormancy at the final stages of development; expression of the LsGA3ox gene occurred at the initial stages of development; the expression of the ACO-B was greater in the initial (5 DAA) and late (20 DAA) stages during development, inducing germination. The expression profile of the LsNCED, LsGA3ox and ACO-B genes was dependent on the cultivar.
URI: http://www.academicjournals.org/journal/AJB/article-full-text-pdf/2DD149622411
http://repositorio.ufla.br/jspui/handle/1/11744
Idioma: en_US
Aparece nas coleções:DAG - Artigos publicados em periódicos

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