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|Título: ||Endo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation|
|Autor(es): ||Santos, F. C.|
Clemente, Aline da Consolação Sampaio
Rosa, Sttela Dellyzete Veiga Franco da
|Publicador: ||Ribeirão Preto Foundation for Research|
|Data de publicação: ||2015|
|Referência: ||SANTOS, F. C. et al. Endo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation. Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 4, p. 11719-11728, 2015.|
|Abstract: ||Coffee seeds begin to develop shortly after fertilization
and can take 6 to 8 months to complete their formation, a period during
which all the characteristics of the mature seed are determined, directly
influencing physiological quality. However, little is known about the
molecular mechanisms that act during coffee seed maturation. The
objective of the current study was to analyze expression of the β-tubulin
(TUB) and endo-β-mannanase (MAN) genes during different phases at
the end of development and in different tissues of Coffea arabica seeds.
The transcription levels of the TUB and MAN genes were quantified in
a relative manner using qRT-PCR in whole seeds, and dissected into
embryos and endosperms at different developmental stages. Greater
expression of MAN was observed in whole seeds and in endosperms
during the green stage, and in the embryo during the over-ripe stage.
High TUB gene expression was observed in whole seeds during the
green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred
in both the green stage and in the cherry stage. These results suggest
participation of endo-β-mannanase during the initial seed developmental
stages, and in the stages of physiological maturity in the embryo tissues.
TUB gene expression varied depending on the developmental stage
and section of seed analyzed, indicating the participation of β-tubulin
during organogenesis and coffee seed maturation.|
|Aparece nas coleções: ||DAG - Artigos publicados em periódicos|
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