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Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/11851

Title: Cryopreservation of Anadenanthera colubrina (Vell.) brenan embryonic axes
???metadata.dc.creator???: Nery, Fernanda Carlota
Paiva, Renato
Campos, A. C. A. L.
Nogueira, Gabriela Ferreira
Stein, Vanesa Cristina
Alvarenga, Amauri Alves de
Keywords: Sementes – Armazenamento
Seeds – Storage
Anadenanthera colubrine
Publisher: International Society for Horticultural Science
Issue Date: Sep-2011
Citation: NERY, F. C. et al. Cryopreservation of Anadenanthera colubrina (Vell.) brenan embryonic axes. Acta Horticulturae, The Hague, v. 908, p. 227-231, Sept. 2011.
Abstract: The ex situ preservation and rational use of Anadenanthera colubrine demand conclusive information related to seed storage. This study aimed determines a protocol for cryopreserving embryonic axes of A. colubrine seeds. Embryonic axes were treated with three concentrations (5, 10, and 15%) of DMSO and glycerol and maintained in liquid nitrogen (-196°C), ultra freezer (-80°C) and freezer (-20°C) for 15, 30, and 150 days. Moisture content of the embryonic axes was determined before and after each storage period. Embryonic axes immersed in chemical cryoprotectants and maintained at -196, -80, and -20°C, absorbed the solutions and had moisture contents higher than 65%. Under these conditions, no germination was observed. Embryonic axes that were stored in the absence of cryoprotectants presented viability around 100%, independently of the cryostorage period.
URI: http://www.actahort.org/books/908/908_28.htm
???metadata.dc.language???: en_US
Appears in Collections:DBI - Artigos publicados em periódicos

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