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|Title: ||Cryopreservation of Anadenanthera colubrina (Vell.) brenan embryonic axes|
|???metadata.dc.creator???: ||Nery, Fernanda Carlota|
Campos, A. C. A. L.
Nogueira, Gabriela Ferreira
Stein, Vanesa Cristina
Alvarenga, Amauri Alves de
|Keywords: ||Sementes – Armazenamento|
Seeds – Storage
|Publisher: ||International Society for Horticultural Science|
|Issue Date: ||Sep-2011|
|Citation: ||NERY, F. C. et al. Cryopreservation of Anadenanthera colubrina (Vell.) brenan embryonic axes. Acta Horticulturae, The Hague, v. 908, p. 227-231, Sept. 2011.|
|Abstract: ||The ex situ preservation and rational use of Anadenanthera colubrine demand conclusive information related to seed storage. This study aimed determines a protocol for cryopreserving embryonic axes of A. colubrine seeds. Embryonic axes were treated with three concentrations (5, 10, and 15%) of DMSO and glycerol and maintained in liquid nitrogen (-196°C), ultra freezer (-80°C) and freezer (-20°C) for 15, 30, and 150 days. Moisture content of the embryonic axes was determined before and after each storage period. Embryonic axes immersed in chemical cryoprotectants and maintained at -196, -80, and -20°C, absorbed the solutions and had moisture contents higher than 65%. Under these conditions, no germination was observed. Embryonic axes that were stored in the absence of cryoprotectants presented viability around 100%, independently of the cryostorage period.|
|Appears in Collections:||DBI - Artigos publicados em periódicos|
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