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dc.creatorViveiros, Ana Tereza de Mendonça-
dc.creatorNascimento, Ariane Flávia do-
dc.creatorOrfão, Laura Helena-
dc.creatorIsaú, Ziara Aparecida-
dc.date.accessioned2016-12-15T18:23:27Z-
dc.date.available2016-12-15T18:23:27Z-
dc.date.issued2010-09-
dc.identifier.citationVIVEIROS, A. T. de M. et al. Motility and fertility of the subtropical freshwater fish streaked prochilod (Prochilodus lineatus) sperm cryopreserved in powdered coconut water. Theriogenology, Stoneham, v. 74, n. 4, p. 551–556, Sept. 2010.pt_BR
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0093691X10001780pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/12077-
dc.description.abstractStreaked prochilod (Prochilodus lineatus) is a freshwater fish inhabiting many South American rivers. The objective was to determine the effectiveness of coconut water (ACP™), combined with methylglycol, as a freezing medium for streaked prochilod sperm. A secondary objective was to compare a computer-assisted sperm analyzer (CASA) system versus subjective microscropic examination as a means of assessing sperm motility. As a control, glucose and methylglycol was used, according to our previous study. Sperm diluted in each medium was loaded into 0.5 mL straws, frozen in liquid nitrogen vapor (in a dry shipper), and stored in liquid nitrogen (-196 °C). Half of the samples were evaluated for sperm motility, both subjectively and with CASA; the remainder were evaluated for fertility. There was no difference (P > 0.05) between subjective or CASA assessment of post-thaw sperm motility. Although sperm motility was higher in sperm cryopreserved in ACP™ (85%) than in glucose (75%), cryopreservation in either extender yielded similar fertilization rates (46–48%) and sperm velocities. There were positive correlations (r = 0.56–0.8) between all sperm velocities and fertilization rate. In conclusion, streaked prochilod sperm cryopreserved in glucose or ACP™ and methylglycol was fertile, and thus could be used for research or commercial settings. Furthermore, although the CASA system provided objective data regarding sperm motility, in the present study, subjective evaluation of sperm motility was practical and a good indication of sperm quality; it could readily be done by well-trained personnel under field or laboratory conditions.pt_BR
dc.languageen_USpt_BR
dc.publisherElsevierpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceTheriogenologypt_BR
dc.subjectProchilodus lineatuspt_BR
dc.subjectSemen cryopreservationpt_BR
dc.subjectSperm motilitypt_BR
dc.subjectFertilitypt_BR
dc.subjectFishpt_BR
dc.titleMotility and fertility of the subtropical freshwater fish streaked prochilod (Prochilodus lineatus) sperm cryopreserved in powdered coconut waterpt_BR
dc.typeArtigopt_BR
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