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|Título :||Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features|
|Autor:||Viveiros, Ana Tereza de Mendonça|
Amaral, Thiciana B
Orfão, Laura Helena
Isaú, Ziara Aparecida
Leal, Marcelo de Castro
|Data da publicação:||Mai-2011|
|Referência:||VIVEIROS, A. T. de M. et al. Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features. Aquaculture Research, Oxford, v. 42, n. 6, p. 858–865, May 2011.|
|Abstract:||The aim of this study was to test the effects of cryoprotectants [dimethyl sulphoxide (DMSO) and methylglycol], extenders (0.9% NaCl, 5% glucose, Beltsville Thawing Solution™ and Merck III™), thawing temperatures (30 and 60 °C) and activating agents (0.29% NaCl and 1% NaHCO3) on the cryopreservation process of tiete tetra Brycon insignis sperm. Sperm was loaded in 0.5 mL straws, frozen in nitrogen vapour at −170 °C and stored in liquid nitrogen. Post-thaw sperm quality was evaluated in terms of subjective motility rate, quality motility score (0=no movement; 5=rapidly swimming spermatozoa), duration of motility and vitality (eosin–nigrosin staining). Post-thaw sperm motility rate was greater in methylglycol (76–88%), compared with DMSO (23–59%). In general, the highest quality motility scores were observed when sperm was thawed at 30 °C and triggered in 1% NaHCO3 (3.5–4.3). Duration of motility was longer when triggered in 1% NaHCO3 (95–120 s) compared with 0.29% NaCl (69–107 s). Sperm vitality was not affected by any of the parameters tested and varied from 51% to 69% intact sperm. Brycon insignis sperm frozen in methylglycol combined with any of the extenders tested and using the methods described above yields motility above 57% and that should last long enough to fertilize oocytes.|
|Aparece nas coleções:||DZO - Artigos publicados em periódicos|
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