Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/12088
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dc.creatorViveiros, Ana Tereza de Mendonça-
dc.creatorMaria, Alexandre Nizio-
dc.creatorAmaral, Thiciana B.-
dc.creatorOrfão, Laura Helena-
dc.creatorIsaú, Ziara Aparecida-
dc.creatorVeríssimo-Silveira, Rosicleire-
dc.date.accessioned2016-12-19T10:26:10Z-
dc.date.available2016-12-19T10:26:10Z-
dc.date.issued2012-03-
dc.identifier.citationVIVEIROS, A. T. de M. et al. Spermatozoon ultrastructure and sperm cryopreservation of the Brazilian dry season spawner fish pirapitinga, Brycon nattereri. Aquaculture Research, Oxford, v. 43, n. 4, p. 546–555, Mar. 2012.pt_BR
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2011.02860.x/abstractpt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/12088-
dc.description.abstractThe aims of this study were to describe the fresh spermatozoon ultrastructure using scanning and transmission electron microscopy and to improve the sperm cryopreservation methodology for the freshwater fish pirapitinga Brycon nattereri. Extenders (BTS™ and NaCl), straw volumes (0.5 and 4.0 mL), thawing temperatures (30 and 60 °C) and activating agents (0.29% NaCl and 1% NaHCO3) were tested. Methylglycol was used as a cryoprotectant agent and sperm was frozen in nitrogen vapour (dry-shipper). Post-thawed sperm motility rate, motility quality (score 0=no movement; 5=rapidly swimming spermatozoa), duration of motility and spermatozoon morphology were evaluated. Fresh spermatozoon was 35.06 μm long, the head was ovoid (2.00 × 1.22 μm) with no acrosome, the midpiece was 2.15 μm long and the flagellum was 30.90 μm long with the typical 9+2 axoneme arrangement. Post-thawed sperm motility rate (70–79% motile sperm), motility quality (score 3.1–3.7) and morphology (9.3–11.6% abnormal spermatozoa) were not affected by any of the parameters tested. The duration of sperm motility was longer when triggered in 1% NaHCO3 (392–1031 s) compared with 0.29% NaCl (144–338 s). Brycon nattereri sperm cryopreserved under the conditions described above yields over 70% motility and should last long enough to fertilize oocytes, even after 2 years of freezing.pt_BR
dc.languageen_USpt_BR
dc.publisherWileypt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceAquaculture Researchpt_BR
dc.titleSpermatozoon ultrastructure and sperm cryopreservation of the Brazilian dry season spawner fish pirapitinga, Brycon nattereript_BR
dc.typeArtigopt_BR
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