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|Título: ||Initiation and suppression of sperm motility is osmolality-dependent in two South American fish species: streaked prochilod (Prochilodus lineatus) and piracanjuba (Brycon orbignyanus)|
|Autor(es): ||Gonçalves, Antônio Carlos Silveira|
Nascimento, Ariane Flávia do
Leal, Marcelo de Castro
Viveiros, Ana Tereza de Mendonça
|Publicador: ||Colégio Brasileiro de Reprodução Animal|
|Data de publicação: ||2013|
|Referência: ||GONÇALVES, A. C. S. et al. Initiation and suppression of sperm motility is osmolality-dependent in two South American fish species: streaked prochilod (Prochilodus lineatus) and piracanjuba (Brycon orbignyanus). Animal Reproduction, Belo Horizonte, v. 10, n. 1, p. 62-70, Jan./Mar. 2013.|
|Abstract: ||Solutions to induce or suppress the initiation of
sperm motility in fish have been used to improve
reproductive success during artificial fertilization and
preservation techniques. The aim of the present study
was to evaluate the effects of three solutions (NaCl,
glucose, and BTS™) - each prepared with 10 different
osmolalities - on the initiation and suppression of fresh
sperm motility in Prochilodus lineatus and Brycon
orbignyanus. Sperm was diluted in each of the 30 solutions
and immediately observed under a light microscope to
determine which solutions triggered or suppressed the
initiation of sperm motility. When present, motility rate
(% motile sperm) was determined at 0, 30, and 120 s
post-activation and the motility quality score ranging
from 0 (no movement) to 5 (rapidly swimming sperm)
was determined at 0 and 30 s post-activation.
Osmolality, but not solution composition, significantly
affected both motility rate and quality score. Solutions
at osmolalities up to 270 mOsm/kg in P. lineatus and up
to 180 mOsm/kg in B. orbignyanus induced motility in
at least 60% of sperm, with a minimum quality score of
3.0, and were therefore classified as activating agents.
The greatest motility at 0, 30, and 120 s post-activation was
observed with solutions ranging from 135 to 225 mOsm/kg
for P. lineatus and at 135 mOsm/kg for B. orbignyanus.
On the other hand, solutions ranging from 360 to
450 mOsm/kg in P. lineatus and 270 to 450 mOsm/kg
in B. orbignyanus suppressed motility in at least 95% of
sperm and were classified as immobilizing media.
The osmolality of the surrounding medium is the key
factor in the initiation or suppression of sperm motility
in P. lineatus and B. orbignyanus|
|Aparece nas coleções: ||DZO - Artigos publicados em periódicos|
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