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metadata.artigo.dc.title: Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment
metadata.artigo.dc.creator: Viveiros, Ana Tereza de Mendonça
Gonçalves, Antônio Carlos Silveira
Di Chiacchio, Isabela Martins
Nascimento, Ariane Flávia do
Romagosa, Elizabeth
Leal, Marcelo de Castro
metadata.artigo.dc.publisher: Cambridge University Press 2015
metadata.artigo.dc.identifier.citation: VIVEIROS, A. T. de M. et al. Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment. Zygote, [S.l.], v. 23, p. 212-221, 2015.
metadata.artigo.dc.description.abstract: The efficiency of OvaprimTM salmon gonadotropin-releasing hormone agonist (GnRHa) and dopamine antagonist on the induction of spawning and spermiation in Prochilodus lineatus in comparison with the commonly used method using pituitary extract (PE) was evaluated. Females received PE at 0.5 + 5.0 mg/kg and OvaprimTM at 0.05 + 0.45 ml/kg or at 0.125 + 0.375 ml/kg. All males received a first dose of PE at 0.4 mg/kg and then PE at 4.0 mg/kg or OvaprimTM at 0.25 ml/kg. Oocyte, egg, larvae and sperm quality were evaluated. All females spawned and oocyte, egg and larvae quality were similar between OvaprimTM-treated (both doses) and PE-treated females. Data from females were pooled and the mean values were: 242 g ova weight, 15% ova index, 1209 oocytes/g ova, 284,539 oocytes/female, 183 oocytes/g body weight, 1.18 mm oocyte diameter, 49% fertilization rate, 43% hatching rate and 89% normal larvae. Sperm quality was similar between OvaprimTM-treated and PE-treated males. Data from males were pooled and the mean values of semen were: volume of 3.0 ml, 14.9 × 109 sperm/ml, osmolality of 283 mOsm/kg, pH of 7.4, 71% motile sperm, 217 μm/s curvilinear velocity, 102 μm/s straight-line velocity and 189 μm/s average path velocity. OvaprimTM treatment can be used for commercial reproduction of P. lineatus, without any loss of gamete quality in comparison with PE treatment.
metadata.artigo.dc.language: en_US
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