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dc.creatorArmesto, Cecilia-
dc.creatorMaia, Fernanda Gonçalves Martins-
dc.creatorAbreu, Mário Sobral de-
dc.creatorFigueira, Antonia dos Reis-
dc.creatorSilva, Bruno Marques da-
dc.creatorMonteiro, Fernando Pereira-
dc.identifier.citationARMESTO, C. et al. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 3, p. 1222-1229, July 2012.pt_BR
dc.description.abstractBlister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.pt_BR
dc.publisherSociedade Brasileira de Microbiologiapt_BR
dc.rightsacesso abertopt_BR
dc.sourceBrazilian Journal of Microbiologypt_BR
dc.subjectColletotrichum gloeosporioidespt_BR
dc.subjectFluorescent proteinspt_BR
dc.titleGenetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spotpt_BR
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