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|Title: ||Characterization of Coffee ringspot virus-Lavras: a model for an emerging threat to coffee production and quality|
|???metadata.dc.creator???: ||Ramalho, T. O.|
Figueira, A. R.
Sotero, A. J.
Geraldino Duarte, P. S.
Goodin, M. M.
Negative-strand RNA virus
|Issue Date: ||Sep-2014|
|Citation: ||RAMALHO, T. O. et al. Characterization of Coffee ringspot virus-Lavras: a model for an emerging threat to coffee production and quality. Virology, [S. l.], v. 464/465, p. 385-396, Sept. 2014.|
|Abstract: ||The emergence of viruses in Coffee (Coffea arabica and Coffea canephora), the most widely traded agricultural commodity in the world, is of critical concern. The RNA1 (6552 nt) of Coffee ringspot virus is organized into five open reading frames (ORFs) capable of encoding the viral nucleocapsid (ORF1p), phosphoprotein (ORF2p), putative cell-to-cell movement protein (ORF3p), matrix protein (ORF4p) and glycoprotein (ORF5p). Each ORF is separated by a conserved intergenic junction. RNA2 (5945 nt), which completes the bipartite genome, encodes a single protein (ORF6p) with homology to RNA-dependent RNA polymerases. Phylogenetic analysis of L protein sequences firmly establishes CoRSV as a member of the recently proposed Dichorhavirus genus. Predictive algorithms, in planta protein expression, and a yeast-based nuclear import assay were used to determine the nucleophillic character of five CoRSV proteins. Finally, the temperature-dependent ability of CoRSV to establish systemic infections in an initially local lesion host was quantified.|
|Appears in Collections:||DFP - Artigos publicados em periódicos|
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