Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/13037
Title: Produção e avaliação de toxicidade de nanomateriais com potencial aplicação da medicina veterinária
Other Titles: Production and evaluation oftoxicity of nanomaterials with potential application in veterinary medicine
Authors: Sousa, Raimundo Vicente de
Brandão, Humberto de Mello
Brandão, Humberto de Mello
Wouters, Flademir
Keywords: Biopolímeros
Staphylococcus aureus
Medicina Veterinária - Nanomateriais
Biopolymers
Veterinary Medicine - Nanomaterials
Issue Date: 23-May-2017
Publisher: Universidade Federal de Lavras
Citation: ABREU, C. P. V. Produção e avaliação de toxicidade de nanomateriais com potencial aplicação da medicina veterinária. 2017. 101 p. Dissertação (Mestrado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2017.
Abstract: Mastitis is one of the principal diseases that affects dairy herds around word. According WHO, Staphylococcus aureus, the main etiological agent of this disease, was recently included in global priority list of antibiotic-resistant bacteria. This pathogen causes extreme threats to health and therefore, requires extensive research and efforts for development of new and effective drugs and vaccines. The biofilm, an important factor of virulence produced by this bacteria, has as the main component the exopolysaccharide (PNAG). Thus, the present research proposes the vibrational spectroscopy evaluation of S. aureus PNAG and its application as non - destructive alternative to determine the degree of polymer deacetylation, and the possible polymer cellular toxicity. The PNAG characterization was performed by nuclear magnetic resonance, Raman, infrared and dynamic light scattering. Biological tests were accomplished using bovine peripheral blood mononuclear cells to evaluate the compound acute toxicity. The polysaccharide was toxic to lymphocytes only at highest concentration (7.5 mg / mL), suggesting that the higher degree of deacetylation, higher the rate of fagocytosis. This study has potential application for the development of immunostimulators for bovine mastitis treatment.
URI: http://repositorio.ufla.br/jspui/handle/1/13037
Appears in Collections:Ciências Veterinárias - Mestrado (Dissertações)



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