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|metadata.artigo.dc.title:||Selective activity of Mucor plumbeus reductase towards (−)-camphorquinone|
|metadata.artigo.dc.creator:||Souza, Giovanni Gontijo de|
Anconi, Cleber Paulo Andrada
Almeida, Wagner Batista de
Santos, Hélio Ferreira dos
Fortes, Isabel Cristina Pereira
Takahashi, Jacqueline Aparecida
|metadata.artigo.dc.identifier.citation:||SOUZA, G. G. de et al. Selective activity of Mucor plumbeus reductase towards (−)-camphorquinone. Journal of Industrial Microbiology & Biotechnology, Hampshire, v. 36, n. 8, p. 1023-1027, Aug. 2009.|
|metadata.artigo.dc.description.abstract:||The biotransformation of 1R-(-)-camphorquinone, achieved by growing cells of four fungi species isolated from soil (Mucor plumbeus, Lecanicillium muscarium, Thamnostylum sp. and Syncephalastrum racemosum), was investigated in optimized culture media for each species. Fungi were grown aerobically under shaking and their activities with respect to camphorquinone were monitored for 20 days by gas chromatography coupled to mass spectrometry (GCMS). Camphorquinone was found to be stable in control flasks throughout the experiment. The most interesting results were found for M. plumbeus, which was only able to perform monoreduction of camphorquinone when cultivated on a glucose-peptone-yeast extract medium. Large-scale experiments were set up and the camphorquinone biotransformation products formed by M. plumbeus were purified by column chromatography and identified by (1)H and (13)C nuclear magnetic resonance (NMR). Theoretical calculations were employed as a complementary technique to unambiguously identify the biotransformation products. These findings suggest that M. plumbeus could be of great use for the selective reduction of camphorquinone and related compounds.|
|Appears in Collections:||DQI - Artigos publicados em periódicos|
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