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Campo DCValorIdioma
dc.creatorDias, Mariana-
dc.creatorMiguel, Maria Gabriela da Cruz Pedrozo-
dc.creatorDuarte, Whasley Ferreira-
dc.creatorSilva, Cristina Ferreira-
dc.creatorSchwan, Rosane Freitas-
dc.date.accessioned2017-08-07T12:43:17Z-
dc.date.available2017-08-07T12:43:17Z-
dc.date.issued2015-06-
dc.identifier.citationDIAS, M. et al. Epiphytic bacteria biodiversity in Brazilian Cerrado fruit and their cellulolytic activity potential. Annals of Microbiology, [S. l.], v. 65, n. 2, p. 851-864, June 2015.pt_BR
dc.identifier.urihttps://link.springer.com/article/10.1007/s13213-014-0927-7pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/15084-
dc.description.abstractThe Cerrado is the second largest Brazilian biome, yet little is known about its wild fauna, flora and microbiota. This work aimed to identify epiphytic bacteria present in fruits native to three different regions of the Cerrado and to select cellulase-producing bacteria. Culture-dependent and culture-independent (PCR-DGGE) methods were used to characterize the microbiota from 32 native Cerrado fruits, and the selection of cellulase-producing bacteria was performed by a semi-quantitative test on carboxymethylcellulose agar medium. Analysis of the 16S rRNA gene sequences of 69 profile representatives showed that the isolates belonged to 29 bacterial genera (Arthrobacter, Bacillus, Paenibacillus, Pseudomonas, Serratia, Staphylococcus, Streptomyces, Enterobacter, Microbacterium, Aerococcus, Bradyrhizobium, Methylobacterium, Erwinia, Pantoea, Acidithiobacillus, Ochrobactrum, Stenotrophomonas, Curtobacterium, Clostridium, Lactobacillus, Xanthomonas, Delftia, Klebsiella, Enterococcus, Burkholderia, Escherichia, Streptococcus, Citrobacter and Achromobacter). Species in the genera Methylobacterium, Stenotrophomonas, Clostridium, Pantoea and Enterobacter were detected by both culture-dependent and culture-independent methods. The species Lactobacillus fermentum, Acinetobacter sp. and Methylomonas methanica were detected only by PCR-DGGE. Additionally, 30 % (178 isolates) of the bacteria tested were able to produce cellulase. The best producers belonged to the genera Bacillus, Streptomyces, Paenibacillus, Enterobacter and Burkholderia, indicating that this ecosystem could be an attractive source for the study of novel enzymes.pt_BR
dc.languageen_USpt_BR
dc.publisherSpringerpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceAnnals of Microbiologypt_BR
dc.subjectBacterial isolationpt_BR
dc.subjectEpiphitic microbiotapt_BR
dc.subjectIsolamento bacterianopt_BR
dc.subjectMicrobiota epífitapt_BR
dc.titleEpiphytic bacteria biodiversity in Brazilian Cerrado fruit and their cellulolytic activity potentialpt_BR
dc.typeArtigopt_BR
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