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dc.creatorMesquita, Vanessa A.-
dc.creatorMachado, Manuela D.-
dc.creatorSilva, Cristina F.-
dc.creatorSoares, Eduardo V.-
dc.date.accessioned2017-08-09T19:27:05Z-
dc.date.available2017-08-09T19:27:05Z-
dc.date.issued2015-07-
dc.identifier.citationMESQUITA, V. A. et al. Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii. Environmental Science and Pollution Research, [S. l.], v. 22, n. 14, p. 11127–11136, July 2015.pt_BR
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs11356-015-4326-9pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/15099-
dc.description.abstractMetal contamination of the environment is frequently associated to the presence of two or more metals. This work aimed to study the impact of a mixture of metals (Cd, Pb and Zn) on the physiology of the non-conventional yeast Pichia kudriavzevii. The incubation of yeast cells with 5 mg/l Cd, 10 mg/l Pb and 5 mg/l Zn, for 6 h, induced a loss of metabolic activity (assessed by FUN-1 staining) and proliferation capacity (evaluated by a clonogenic assay), with a small loss of membrane integrity (measured by trypan blue exclusion assay). The staining of yeast cells with calcofluor white revealed that no modification of chitin deposition pattern occurred during the exposure to metal mixture. Extending for 24 h, the exposure of yeast cells to metal mixture provoked a loss of membrane integrity, which was accompanied by the leakage of intracellular components. A marked loss of the metabolic activity and the loss of proliferation capacity were also observed. The analysis of the impact of a single metal has shown that, under the conditions studied, Pb was the metal responsible for the toxic effect observed in the metal mixture. Intracellular accumulation of Pb seems to be correlated with the metals’ toxic effects observed.pt_BR
dc.languageen_USpt_BR
dc.publisherSpringerpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceEnvironmental Science and Pollution Researchpt_BR
dc.subjectCell deadpt_BR
dc.subjectMetal contaminationpt_BR
dc.subjectMetabolic activity inhibitionpt_BR
dc.subjectMetals toxicitypt_BR
dc.subjectYeast survivalpt_BR
dc.subjectMorte celularpt_BR
dc.subjectContaminação por metaispt_BR
dc.subjectInibição da atividade metabólicapt_BR
dc.subjectToxicidade nos metaispt_BR
dc.subjectSobrevida de fermentopt_BR
dc.titleImpact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzeviipt_BR
dc.typeArtigopt_BR
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