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dc.creatorJaramillo, Paula Marcela Duque-
dc.creatorGuimarães, Amanda Azarias-
dc.creatorFlorentino, Ligiane Aparecida-
dc.creatorSilva, Karina Barroso-
dc.creatorNóbrega, Rafaela Simão Abrahão-
dc.creatorMoreira, Fatima Maria de Souza-
dc.identifier.citationJARAMILLO, P. M. D. et al. Symbiotic nitrogen-fixing bacterial populations trapped from soils under agroforestry systems in the Western Amazon. Scientia Agricola, Piracicaba, v. 70, n. 6, p. 397-404, Nov./Dec. 2013.pt_BR
dc.description.abstractCowpea (Vigna unguiculata) is an important grain-producing legume that can forego nitrogen fertilization by establishing an efficient symbiosis with nitrogen-fixing bacteria. Although inoculating strains have already been selected for this species, little is known about the genotypic and symbiotic diversity of native rhizobia. Recently, Bradyrhizobium has been shown to be the genus most frequently trapped by cowpea in agricultural soils of the Amazon region. We investigated the genetic and symbiotic diversity of 148 bacterial strains with different phenotypic and cultural properties isolated from the nodules of the trap species cowpea, which was inoculated with samples from soils under agroforestry systems from the western Amazon. Sixty non-nodulating strains indicated a high frequency of endophytic strains in the nodules. The 88 authenticated strains had varying symbiotic efficiency. The SPAD (Soil Plant Analysis Development) index (indirect measurement of chlorophyll content) was more efficient at evaluating the contribution of symbiotic N2-fixation than shoot dry matter under axenic conditions. Cowpea-nodulating bacteria exhibited a high level of genetic diversity, with 68 genotypes identified by BOX-PCR. Sequencing of the 16S rRNA gene showed a predominance of the genus Bradyrhizobium, which accounted for 70 % of all strains sequenced. Other genera identified were Rhizobium, Ochrobactrum, Paenibacillus, Bosea, Bacillus, Enterobacter, and Stenotrophomonas. These results support the promiscuity of cowpea and demonstrate the high genetic and symbiotic diversity of rhizobia in soils under agroforestry systems, with some strains exhibiting potential for use as inoculants. The predominance of Bradyrhizobium in land uses with different plant communities and soil characteristics reflects the adaptation of this genus to the Amazon region.pt_BR
dc.publisherEscola Superior de Agricultura "Luiz de Queiroz"pt_BR
dc.rightsacesso abertopt_BR
dc.rightsAttribution-NonCommercial 4.0 International*
dc.sourceScientia Agricolapt_BR
dc.subjectLegume-nodulating bacteriapt_BR
dc.subjectNitrogen fertilizationpt_BR
dc.subjectNitrogen-fixing bacteriapt_BR
dc.subjectSymbiotic promiscuitypt_BR
dc.subjectChlorophyll contentpt_BR
dc.subjectBactérias nodulantes de leguminosaspt_BR
dc.subjectAdubação nitrogenadapt_BR
dc.subjectBactérias fixadoras de nitrogêniopt_BR
dc.subjectPromiscuidade simbióticapt_BR
dc.subjectConteúdo de clorofilapt_BR
dc.titleSymbiotic nitrogen-fixing bacterial populations trapped from soils under agroforestry systems in the Western Amazonpt_BR
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