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dc.creatorMesquita, Vanessa A.-
dc.creatorSilva, Cristina F.-
dc.creatorSoares, Eduardo V.-
dc.date.accessioned2018-07-13T11:15:42Z-
dc.date.available2018-07-13T11:15:42Z-
dc.date.issued2016-05-
dc.identifier.citationMESQUITA, V. A.; SILVA, C. F.; SOARES, E. V. Toxicity induced by a metal mixture (Cd, Pb and Zn) in the yeast Pichia kudriavzevii: the role of oxidative stress. Current Microbiology, New York, v. 72, n. 5, p. 545-550, May 2016.pt_BR
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00284-016-0987-ypt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/29624-
dc.description.abstractThe present work aims to contribute for the elucidation of the role of oxidative stress in the toxicity associated with the exposure of Pichia kudriavzevii to multi-metals (Cd, Pb and Zn). Cells of the non-conventional yeast P. kudriavzevii exposed for 6 h to the action of multi-metals accumulated intracellular reactive oxygen species (ROS), evaluated through the oxidation of the probe 2′,7′-dichlorodihydrofluorescein diacetate. A progressive loss of membrane integrity (monitored using propidium iodide) was observed in multi-metal-treated cells. The triggering of intracellular ROS accumulation preceded the loss of membrane integrity. These results suggest that the disruption of membrane integrity can be attributed to the oxidative stress. The exposure of yeast cells to single metal showed that, under the concentrations tested, Pb was the metal responsible for the induction of the oxidative stress. Yeast cells coexposed to an antioxidant (ascorbic acid) and multi-metals did not accumulate intracellular ROS, but loss proliferation capacity. Together, the data obtained indicated that intracellular ROS accumulation contributed to metal toxicity, namely for the disruption of membrane integrity of the yeast P. kudriavzevii. It was proposed that Pb toxicity (the metal responsible for the toxic symptoms under the conditions tested) result from the combination of an ionic mechanism and the intracellular ROS accumulation.pt_BR
dc.languageen_USpt_BR
dc.publisherSpringerpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceCurrent Microbiologypt_BR
dc.subjectReactive oxygen speciespt_BR
dc.subjectYeast cellpt_BR
dc.subjectMembrane integritypt_BR
dc.subjectIntracellular reactive oxygen speciespt_BR
dc.subjectProliferation capacitypt_BR
dc.subjectEspécies que reagem ao oxigêniopt_BR
dc.subjectCélula de levedurapt_BR
dc.subjectIntegridade da membranapt_BR
dc.subjectEspécies reativas intracelulares de oxigêniopt_BR
dc.titleToxicity induced by a metal mixture (Cd, Pb and Zn) in the yeast Pichia kudriavzevii: the role of oxidative stresspt_BR
dc.typeArtigopt_BR
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