Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/31412
metadata.artigo.dc.title: Effect of SPL (Spent Pot Liner) and its main components on root growth, mitotic activity and phosphorylation of Histone H3 in Lactuca sativa L.
metadata.artigo.dc.creator: Freitas, Aline Silva
Cunha, Isabela Martinez Fontes
Vieira, Larissa Fonseca Andrade
Techio, Vânia Helena
metadata.artigo.dc.subject: Histone H3 serine 10 phosphorylation (H3S10ph)
Cellular change
Fragile sites
Chromosomal alterations
Micronuclei
Spent Pot Liner (SPL)
metadata.artigo.dc.publisher: Elsevier
metadata.artigo.dc.date.issued: Feb-2016
metadata.artigo.dc.identifier.citation: FREITAS, A. S. et al. Effect of SPL (Spent Pot Liner) and its main components on root growth, mitotic activity and phosphorylation of Histone H3 in Lactuca sativa L. Ecotoxicology and Environmental Safety, [S.l], v. 124, p. 426-434, Feb. 2016.
metadata.artigo.dc.description.abstract: Spent Pot Liner (SPL) is a solid waste from the aluminum industry frequently disposed of in industrial landfills; it can be leached and contaminate the soil, sources of drinking water and plantations, and thus may pose a risk to human health and to ecosystems. Its composition is high variable, including cyanide, fluoride and aluminum salts, which are highly toxic and environmental pollutants. This study evaluated the effect of SPL and its main components on root growth and the mitosis of Lactuca sativa, by investigating the mechanisms of cellular and chromosomal alterations with the aid of immunolocalization. To this end, newly emerged roots of L. sativa were exposed to SPL and its main components (solutions of cyanide, fluoride and aluminum) and to calcium chloride (control) for 48 h. After this, root length was measured and cell cycle was examined by means of conventional cytogenetics and immunolocalization. Root growth was inhibited in the treatments with SPL and aluminum; chromosomal and nuclear alterations were observed in all treatments. The immunolocalization evidenced normal dividing cells with regular temporal and spatial distribution of histone H3 phosphorylation at serine 10 (H3S10ph). However, SPL and its main components inhibited the phosphorylation of histone H3 at serine 10, inactivated pericentromeric regions and affected the cohesion of sister chromatids, thus affecting the arrangement of chromosomes in the metaphase plate and separation of chromatids in anaphase. In addition, these substances induced breaks in pericentromeric regions, characterized as fragile sites.
metadata.artigo.dc.identifier.uri: https://www.sciencedirect.com/science/article/pii/S0147651315301640
http://repositorio.ufla.br/jspui/handle/1/31412
metadata.artigo.dc.language: en_US
Appears in Collections:DBI - Artigos publicados em periódicos

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