Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/31716
Título: Immobilization of sugars in supermacroporous cryogels for the purification of lectins by affinity chromatography
Palavras-chave: Cryogel
Affinity chromatography
Ligands
Monoliths
Cromatografia de afinidade
Ligandos
Monólitos
Data do documento: 15-Nov-2017
Editor: Elsevier
Citação: GONÇALVES, G. R. F. et al. Immobilization of sugars in supermacroporous cryogels for the purification of lectins by affinity chromatography. Journal of Chromatography B, Amsterdam, v. 1068-1069, p. 71-77, 15 Nov. 2017.
Resumo: Lectins are glycoproteins that bind to carbohydrates or glycoconjugates by specific interactions. The specificity of lectins to various carbohydrates is a determinant factor in the choice of ligand for the chromatographic matrix when using chromatography as a lectin purification technique. In this work, the immobilization of three different aminated carbohydrates on the surface of macroporous polymeric cryogels was evaluated. Carbohydrates were immobilized on cryogel surfaces via the glutaraldehyde method to create spacer arms, reducing steric hindrance. The immobilized N-acetyl-d-glucosamine and N-acetyl-d-mannosamine concentrations contained approximately 130 mg of carbohydrate/g dehydrated cryogel, while the N-acetyl-d-galactosamine contained 105 mg of carbohydrate/g dehydrated cryogel. Scanning electron microscopy showed that the physical structure and porosity of the chromatographic columns were not affected by the immobilization process, maintaining an elevated hydration capacity and the macroporous structure of the cryogels. Adsorption of concanavalin A on cryogels functionalized with N-acetyl-d-glucosamine (cryo-d-GlcNAc) was tested, as well as its reuse capability. After 5 cycles of use, cryo-d-GlcNAc was shown to be stable, with an adsorptive capacity of around 50 mg/g. Carbohydrate immobilization in polyacrylamide cryogels was satisfactory, with promise for applications in lectin purification processes.
URI: https://www.sciencedirect.com/science/article/pii/S1570023217313193?via%3Dihub#!
http://repositorio.ufla.br/jspui/handle/1/31716
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