Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/31931
metadata.artigo.dc.title: Illumina sequencing-based analysis of a microbial community enriched under anaerobic methane oxidation condition coupled to denitrification revealed coexistence of aerobic and anaerobic methanotrophs
metadata.artigo.dc.creator: Siniscalchi, Luciene Alves Batista
Leite, Laura Rabelo
Oliveira, Guilherme
Chernicharo, Carlos Augusto Lemos
Araújo, Juliana Calabria de
metadata.artigo.dc.subject: Damo process
Illumina sequencing
Sewage sludge
Sequencing batch reactor
Processo Damo
Sequenciamento Illumina
Lodo de esgoto
Reator em lote de sequenciamento
metadata.artigo.dc.publisher: Springer
metadata.artigo.dc.date.issued: Jul-2017
metadata.artigo.dc.identifier.citation: SINISCALCHI, L. A. B. et al. Illumina sequencing-based analysis of a microbial community enriched under anaerobic methane oxidation condition coupled to denitrification revealed coexistence of aerobic and anaerobic methanotrophs. Environmental Science and Pollution Research, [S. l.], v. 24, n. 20, p. 16751-16764, July 2017.
metadata.artigo.dc.description.abstract: Methane is produced in anaerobic environments, such as reactors used to treat wastewaters, and can be consumed by methanotrophs. The composition and structure of a microbial community enriched from anaerobic sewage sludge under methane-oxidation condition coupled to denitrification were investigated. Denaturing gradient gel electrophoresis (DGGE) analysis retrieved sequences of Methylocaldum and Chloroflexi. Deep sequencing analysis revealed a complex community that changed over time and was affected by methane concentration. Methylocaldum (8.2%), Methylosinus (2.3%), Methylomonas (0.02%), Methylacidiphilales (0.45%), Nitrospirales (0.18%), and Methanosarcinales (0.3%) were detected. Despite denitrifying conditions provided, Nitrospirales and Methanosarcinales, known to perform anaerobic methane oxidation coupled to denitrification (DAMO) process, were in very low abundance. Results demonstrated that aerobic and anaerobic methanotrophs coexisted in the reactor together with heterotrophic microorganisms, suggesting that a diverse microbial community was important to sustain methanotrophic activity. The methanogenic sludge was a good inoculum to enrich methanotrophs, and cultivation conditions play a selective role in determining community composition.
metadata.artigo.dc.identifier.uri: https://link.springer.com/article/10.1007%2Fs11356-017-9197-9
http://repositorio.ufla.br/jspui/handle/1/31931
metadata.artigo.dc.language: en_US
Appears in Collections:DEG - Artigos publicados em periódicos

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