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|metadata.artigo.dc.title:||Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity|
|metadata.artigo.dc.creator:||Riske, Karin A.|
Domingues, Cleyton C.
Casadei, Bruna R.
Caritá, Amanda C.
Lira, Rafael B.
Preté, Paulo S. C
Paula, Eneida de
Giant unilamellar vesicles
Erythrocyte membrane domains
Solubilização de biomembranas
Vesículas unilamelares gigantes
Separação de fase
Domínios da membrana eritrocitária
|metadata.artigo.dc.identifier.citation:||RISKE, K. A. et al. Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity. Biophysical Reviews, [S. l.], v. 9, n. 5, p. 649-667, Oct. 2017.|
|metadata.artigo.dc.description.abstract:||Detergents are amphiphilic molecules widely used to solubilize biological membranes and/or extract their components. Nevertheless, because of the complex composition of biomembranes, their solubilization by detergents has not been systematically studied. In this review, we address the solubilization of erythrocytes, which provide a relatively simple, robust and easy to handle biomembrane, and of biomimetic models, to stress the role of the lipid composition on the solubilization process. First, results of a systematic study on the solubilization of human erythrocyte membranes by different series of non-ionic (Triton, CxEy, Brij, Renex, Tween), anionic (bile salts) and zwitterionic (ASB, CHAPS) detergents are shown. Such quantitative approach allowed us to propose Re sat—the effective detergent/lipid molar ratio in the membrane for the onset of hemolysis as a new parameter to classify the solubilization efficiency of detergents. Second, detergent-resistant membranes (DRMs) obtained as a result of the partial solubilization of erythrocytes by TX-100, C12E8 and Brij detergents are examined. DRMs were characterized by their cholesterol, sphingolipid and specific proteins content, as well as lipid packing. Finally, lipid bilayers of tuned lipid composition forming liposomes were used to investigate the solubilization process of membranes of different compositions/phases induced by Triton X-100. Optical microscopy of giant unilamellar vesicles revealed that pure phospholipid membranes are fully solubilized, whereas the presence of cholesterol renders the mixture partially or even fully insoluble, depending on the composition. Additionally, Triton X-100 induced phase separation in raft-like mixtures, and selective solubilization of the fluid phase only.|
|Appears in Collections:||DQI - Artigos publicados em periódicos|
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