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|metadata.artigo.dc.title:||Daily rhythms of the expression of genes from the somatotropic axis: the influence on tilapia (Oreochromis niloticus) of feeding and growth hormone administration at different times|
|metadata.artigo.dc.creator:||Costa, Leandro S.|
Rosa, Priscila V.
Sánchez-Vázquez, F. Javier
López-Olmeda, Jose F.
|metadata.artigo.dc.subject:||Pituitary adenylate cyclase-activating polypeptide|
Insulin-like growth factor
Growth hormone receptor
Polipeptídeo ativador da adenilato ciclase pituitária
Hormonio de crescimento
Fator de crescimento semelhante à insulina
Receptor de hormônio de crescimento
Receptor de IGF
Cronofarmacologia do GH
|metadata.artigo.dc.identifier.citation:||COSTA, L. S. et al. Daily rhythms of the expression of genes from the somatotropic axis: the influence on tilapia (Oreochromis niloticus) of feeding and growth hormone administration at different times. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, New York, v. 181-182, p. 27-34, Mar./Apr. 2016.|
|metadata.artigo.dc.description.abstract:||The aim of this research was to investigate the presence of daily rhythms in the somatotropic axis of tilapia fed at two times (mid-light, ML or mid-dark, MD) and the influence of the time of day of growth hormone (GH) administration on the response of this axis. Two different GH injection times were tested: ZT 3 (3 h after lights on) and ZT 15 (3 h after lights off). In both experiments, the mRNA expression levels of hypothalamic pituitary adenylate cyclase-activating polypeptide (pacap), pituitary growth hormone (gh), liver insulin-like growth factors (igf1 and igf2a), and liver and muscle growth hormone receptors (ghr1 and ghr2) and IGF receptors (igf1ra and igf2r) were evaluated by means of qPCR. Daily rhythms were observed in the liver for ghr1, ghr2 and igf2r but only in fish fed at ML, with the acrophases located in the light phase (ZT 3:30, 3:31 and 7:38 h, respectively). In the muscle, ghr1 displayed a significant rhythm in both groups and ghr2 in ML fed fish (acrophases at ZT 5:29, 7:14 and 9:23 h). The time of both GH administration and feeding influenced the response to GH injection: ML fed fish injected with GH at ZT 15 h showed a significant increase in liver igf1, igf2a and ghr2; and muscle ghr2 expression. This is the first report that describes the existence of daily rhythms in the somatotropic axis of tilapia and its time-dependent responses of GH administration. Our results should be considered when investigating the elements of the somatotropic axis in tilapia and GH administration.|
|Appears in Collections:||DZO - Artigos publicados em periódicos|
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