Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/33241
metadata.artigo.dc.title: Haploid identification using tropicalized haploid inducer progenies in maize
metadata.artigo.dc.creator: Ribeiro, Camila Bastos
Pereira, Francielly de Cássia
Nóbrega Filho, Lamartine da
Rezende, Breno Alvarenga
Dias, Kaio Olímpio das Graças
Braz, Guilherme Tomaz
Ruy, Murilo Candido
Silva, Maria Beatriz
Cenzi, Gisele
Techio, Vânia Helena
Souza, João Cândido de
metadata.artigo.dc.subject: Flow cytometry
Zea mays
Chromosome counting
Chromosome duplication
Ploidy level
metadata.artigo.dc.publisher: Universidade Federal de Viçosa (UFV), Departamento de Fitotecnia (DFT)
metadata.artigo.dc.date.issued: Mar-2018
metadata.artigo.dc.identifier.citation: RIBEIRO, C. B. et al. Haploid identification using tropicalized haploid inducer progenies in maize. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 1, p. 16-23, Jan./Mar. 2018. DOI: 10.1590/1984-70332018v18n1a3.
metadata.artigo.dc.description.abstract: The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants.
metadata.artigo.dc.identifier.uri: http://repositorio.ufla.br/jspui/handle/1/33241
metadata.artigo.dc.language: en_US
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