Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/33414
metadata.artigo.dc.title: Bradyrhizobium forestalis sp. nov., an efficient nitrogen-fixing bacterium isolated from nodules of forest legume species in the Amazon
metadata.artigo.dc.creator: Costa, Elaine Martins da
Guimarães, Amanda Azarias
Carvalho, Teotonio Soares de
Rodrigues, Tainara Louzada
Ribeiro, Paula Rose de Almeida
Lebbe, Liesbeth
Willems, Anne
Moreira, Fatima M. de Souza
metadata.artigo.dc.subject: Bradyrhizobium
Biological nitrogen fixation
Polyphasic taxonomy
Symbiotic genes
Fixação biológica de nitrogênio
Taxonomia polifásica
Genes simbióticos
metadata.artigo.dc.publisher: Springer
metadata.artigo.dc.date.issued: Jul-2018
metadata.artigo.dc.identifier.citation: COSTA, E. M. da et al. Bradyrhizobium forestalis sp. nov., an efficient nitrogen-fixing bacterium isolated from nodules of forest legume species in the Amazon. Archives of Microbiology, Berlin, v. 200, n. 5, p. 743-752, July 2018.
metadata.artigo.dc.description.abstract: Three strains of nitrogen-fixing bacteria isolated from nodules of Inga sp. (INPA54BT) and Swartzia sp. (INPA86A and INPA01-91A) in soils under native forest in the Brazilian Amazon were previously identified as belonging to the Bradyrhizobium genus. In this study, these strains were characterized using a polyphasic approach to establish their taxonomic position. The three strains shared more than 99.5% sequence similarity of the 16S rRNA gene with the type strains of five Bradyrhizobium species (B. japonicum USDA 6T, B. liaoningense LMG 18230T, B. ottawaense OO99T, B. subterraneum 58 2-1T and B. yuanmingense LMG 21827T). However, multilocus sequence analysis of two (recA and glnII) or three (atpD, gyrB, and recA) housekeeping genes indicated that these three strains represent a new Bradyrhizobium species, which is closely related to B. subterraneum 58 2-1T and B. yuanmingense LMG 21827T. DNA–DNA hybridization values between INPA54BT and B. subterraneum 58 2-1T and B. yuanmingense LMG 21827T were only 41.5 and 30.9%, respectively. Phenotypic characterization also allowed the differentiation of the novel species from B. subterraneum 58 2-1T and B. yuanmingense LMG 21827T. In the phylogenetic analysis of the nodC and nifH genes, the three strains showed similar sequences that were divergent from those of type strains of all Bradyrhizobium species. We concluded that these strains represent a novel species, for which the name Bradyrhizobium forestalis is proposed, with INPA54BT (= LMG 10044T) as type strain. The G+C content in the DNA of INPA54BT is 63.7 mol%.
metadata.artigo.dc.identifier.uri: https://link.springer.com/article/10.1007%2Fs00203-018-1486-2
http://repositorio.ufla.br/jspui/handle/1/33414
metadata.artigo.dc.language: en_US
Appears in Collections:DCS - Artigos publicados em periódicos

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