Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/34166
metadata.artigo.dc.title: Liposomal-based lidocaine formulation for the improvement of infiltrative buccal anesthesia
metadata.artigo.dc.creator: Almeida, Ana Cláudia Pedreira de
Pinto, Luciana Matos Alves
Alves, Giuliana Piovesan
Ribeiro, Lígia Nunes de Morais
Santana, Maria Helena Andrade
Cereda, Cíntia Maria Saia
Fraceto, Leonardo Fernandes
Paula, Eneida de
metadata.artigo.dc.subject: Liposomes
Lidocaine
Infraorbital nerve blockade
Hydrogenated soybean phosphatidylcholine
Drug-delivery
Dentistry
metadata.artigo.dc.publisher: Taylor and Francis Online
metadata.artigo.dc.date.issued: 2019
metadata.artigo.dc.identifier.citation: ALMEIDA, A. C. P. de et al. Liposomal-based lidocaine formulation for the improvement of infiltrative buccal anesthesia. Journal of Liposome Research, [S.l.], v. 29, n. 1, 2019.
metadata.artigo.dc.description.abstract: This study describes the encapsulation of the local anaesthetic lidocaine (LDC) in large unilamellar liposomes (LUV) prepared in a scalable procedure, with hydrogenated soybean phosphatidylcholine, cholesterol and mannitol. Structural properties of the liposomes were assessed by dynamic light scattering, nanoparticle tracking analysis and transmission electron microscopy. A modified, two-compartment Franz-cell system was used to evaluate the release kinetics of LDC from the liposomes. The in vivo anaesthetic effect of liposomal LDC 2% (LUVLDC) was compared to LDC 2% solution without (LDCPLAIN) or with the vasoconstrictor epinephrine (1:100 000) (LDCVASO), in rat infraorbital nerve blockade model. The structural characterization revealed liposomes with spherical shape, average size distribution of 250 nm and low polydispersity even after LDC incorporation. Zeta potential laid around –30 mV and the number of suspended liposomal particles was in the range of 1012 vesicles/mL. Also the addition of cryoprotectant (mannitol) did not provoke structural changes in liposomes properties. In vitro release profile of LDC from LUV fits well with a biexponential model, in which the LDC encapsulated (EE% = 24%) was responsible for an increase of 67% in the release time in relation to LDCPLAIN (p < 0.05). Also, the liposomal formulation prolonged the sensorial nervous blockade duration (∼70 min), in comparison with LDCPLAIN (45 min), but less than LDCVASO (130 min). In this context, this study showed that the liposomal formulations prepared by scalable procedure were suitable to promote longer and safer buccal anaesthesia, avoiding side effects of the use of vasoconstrictors.
metadata.artigo.dc.identifier.uri: https://www.tandfonline.com/doi/abs/10.1080/08982104.2018.1483947
http://repositorio.ufla.br/jspui/handle/1/34166
metadata.artigo.dc.language: en_US
Appears in Collections:DQI - Artigos publicados em periódicos

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