Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/34454
metadata.artigo.dc.title: Microcosm environment models for studying the stability of adenovirus and murine norovirus in water and sediment
metadata.artigo.dc.creator: Elmahdy, M. E. I.
Magri, M. E.
Garcia, L. A.
Fongaro, G.
Barardi, C. R. M.
metadata.artigo.dc.subject: Enteric viruses
Recombinant adenovirus
Surrogate
metadata.artigo.dc.publisher: Elsevier
metadata.artigo.dc.date.issued: May-2018
metadata.artigo.dc.identifier.citation: ELMAHDY, M. E. I. et al. Microcosm environment models for studying the stability of adenovirus and murine norovirus in water and sediment. International Journal of Hygiene and Environmental Health, [S.l.], v. 221, n. 4, p. 734-741, May 2018.
metadata.artigo.dc.description.abstract: Microcosms are useful tools for understanding the survival and fate of enteric viruses in aquatic environments. This study set out to determine the stability of infectious enteric viruses in an aquatic environment using a laboratory scale microcosm. Sediment and overlaying water were collected from a lagoon and inoculated with known concentrations of recombinant adenovirus (AdV-GFP) and murine norovirus (MNV-1). Infectious particles of these viruses were measured using fluorescence microscopy (AdV-GFP) or the plaque assay method (MNV-1), over 85 days in two different conditions: under natural sunlight and in fully darkened environments. The time required to reach one log reduction in viral titres (T90) of viable viruses in a natural condition microcosm for AdV-GFP and MNV-1 was shorter than in a dark condition microcosm. There was also a negative correlation between the temperature and infectivity of these viruses in both water and sediment samples. Considering that microcosms aim to mimic natural environment conditions and that AdV-GFP and MNV-1 are excellent surrogates for measuring the infectivity of the respective viruses strains, the results presented here have the potential to be applied in future health hazard studies and also would be useful for future climate scenarios.
metadata.artigo.dc.identifier.uri: https://www.sciencedirect.com/science/article/pii/S1438463917307319
http://repositorio.ufla.br/jspui/handle/1/34454
metadata.artigo.dc.language: en_US
Appears in Collections:DEG - Artigos publicados em periódicos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.