Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/34608
Registro completo de metadados
Campo DCValorIdioma
dc.creatorFerreira, Marco Tulio Mendes-
dc.creatorRocha, Laiane Corsini-
dc.creatorVitoriano, Matheus Braga Zanon-
dc.creatorMittelmann, Andréa-
dc.creatorTechio, Vânia Helena-
dc.date.accessioned2019-06-04T15:43:52Z-
dc.date.available2019-06-04T15:43:52Z-
dc.date.issued2018-12-
dc.identifier.citationFERREIRA, M. T. M. et al. Relationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex. Molecular Biology Reports, [S.l.], v. 45, n. 6, p. 1663-1679, Dec. 2018. DOI: 10.1007/s11033-018-4310-9.pt_BR
dc.identifier.urihttps://link.springer.com/article/10.1007/s11033-018-4310-9pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/34608-
dc.description.abstractThe grasses of the Lolium–Festuca complex show a prominent role in world agricultural scenario. Several studies have demonstrated that the plasticity of 45S rDNA sites has been recently associated with the possible fragility of the loci. Often, these fragile sites were observed as extended sites and gaps in metaphases. This organization can be evaluated in relation to their transcriptional activity/accessibility through epigenetic changes. Thus, this study aimed to investigate the relationship of the 5-methylcytosine and histone H3 lysine-9 dimethylation in different conformations of 45S rDNA sites in interphase nuclei and in metaphase chromosomes of L. perenne, L. multiflorum and F. arundinacea. The FISH technique using 45S rDNA probes was performed sequentially after the immunolocalization. The sites showed predominantly the following characteristics in the interphase nuclei: intra- and perinucleolar position, decondensed or partially condensed and hypomethylated and hyper/hypomethylated status. Extranucleolar sites were mainly hypermethylated for both epigenetic marks. The 45S rDNA sites with gaps identified in metaphases were always hypomethylated, which justifies it decondensed and transcriptional state. The frequency of sites with hypermethylated gaps was very low. The structural differences observed in these sites are directly related to the assessed epigenetic marks, justifying the different conformations throughout the cell cycle.pt_BR
dc.languageen_USpt_BR
dc.publisherSpringerpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceMolecular Biology Reportspt_BR
dc.subjectEpigenetic markspt_BR
dc.subjectLolium-Festuca complexpt_BR
dc.subjectPost translational modificationpt_BR
dc.subjectFragile sitespt_BR
dc.subjectPost-translational modification (PTM)pt_BR
dc.titleRelationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complexpt_BR
dc.typeArtigopt_BR
Aparece nas coleções:DBI - Artigos publicados em periódicos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.