Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/34761
metadata.artigo.dc.title: The protective effect exerted by ascorbic acid on DNA fragmentation of human leukocytes induced by Lachesis muta muta venom
metadata.artigo.dc.creator: Trento, Marcus Vinícius Cardoso
Eleutério, Mateus William de Faria
Abreu, Tatiane Silva
Machado, Gustavo Henrique Andrade
Cesar, Pedro Henrique Souza
Simão, Anderson Assaid
Marcussi, Silvana
metadata.artigo.dc.subject: Comet assay
Genotoxicity
Micronucleus
Snake venom
metadata.artigo.dc.publisher: Wiley
metadata.artigo.dc.date.issued: Mar-2019
metadata.artigo.dc.identifier.citation: TRENTO, M. V. C. et al. The protective effect exerted by ascorbic acid on DNA fragmentation of human leukocytes induced by Lachesis muta muta venom. Journal of Cellular Biochemistry, [S.l.], v. 120, n. 3, p. 3520-3528, Mar. 2019.
metadata.artigo.dc.description.abstract: The objective of this study was to evaluate the genotoxic and mutagenic effects of the toxins present in Lachesis muta muta's venom on human peripheral blood leukocytes and the protective potential of ascorbic acid on DNA fragmentation. The venom of L. muta muta was incubated in different concentrations (1, 2.5, 5, 7.5, 10, 15, 20, 30, 40, 50, 60, and 120 µg/mL) with human blood to evaluate DNA fragmentation using the comet, agarose gel electrophoresis, and micronucleus assays. In these concentrations evaluated, the venom of L. muta muta induced genotoxicity (comet assay and agarose gel electrophoresis) and mutagenicity (micronucleus test), but they were not cytotoxic, as they did not change the rate of cell proliferation after cytokinesis blockade with cytochalasin B. The ascorbic acid significantly inhibited the genotoxicity induced by L. muta muta venom in the proportions evaluated (1:0.1 and 1:0.5, venom/ascorbic acid ‐ w/w). Thus, future studies are needed to elucidate the protective mechanisms of ascorbic acid on the genotoxic effects induced by toxins present in snake venoms.
metadata.artigo.dc.identifier.uri: https://onlinelibrary.wiley.com/doi/10.1002/jcb.27628
http://repositorio.ufla.br/jspui/handle/1/34761
metadata.artigo.dc.language: en_US
Appears in Collections:DQI - Artigos publicados em periódicos

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