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Title: Dilute-and-shoot and quechers modified in house for determination of multimycotoxins in cocoa
Keywords: Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Food safety
Sample preparation
Issue Date: 2018
Citation: ABREU, D. C. P. et al. Dilute-and-shoot and quechers modified in house for determination of multimycotoxins in cocoa. In: BRAZILIAN CONFERENCE ON MASS SPECTROMETRY, 7.; BRAZILIAN PROTEOMICS SOCIETY CONFERENCE, 4., 2018, Rio de Janeiro. Conference Book… [S.l.]: [s.n.], 2018. p. 70.
Abstract: Due to the high complexity of the cocoa matrices, a modification to the dilute-and-shoot and QuEChERS methods was proposed for the simultaneous analysis of 42 mycotoxins. The modification allowed an efficient extraction with reduced co-extractives of the matrix without clean up step with LCMS/MS analysis. The ideal condition was: extraction of the cocoa paste with a ratio of 6:4 ACN/H2O (v/v), followed by partitioning of the extractive phase with addition of 3g of NaCl, stirring on a shaker table for 60min and freezing for 15min at -70ºC, the mixture was centrifuged and the supernatant collected for analysis. The performance of the proposed method was evaluated for 35 mycotoxins, considering the recovery range of 70-120% and RSD less than 20%, 5 mycotoxins presented an unsatisfactory result one of the levels of fortification. LD's ranged in between 1.3-27.6μg kg-1. The limits of aflatoxins and ochratoxins defined in the legislation for cocoa were met.
Appears in Collections:DQI - Trabalhos apresentados em eventos

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