Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/38059
Title: Micropropagação do maracujazeiro-do-sono
Other Titles: Micropropagation of Passiflora setacea DC.
Keywords: Passiflora setacea DC.
Maracujá
Cultura de tecidos
In vitro
Passion fruit
Tissue culture
Issue Date: Feb-2010
Publisher: Universidade Federal de Viçosa (UFV)
Citation: SANTOS, F. C. et al. Micropropagação do maracujazeiro-do-sono. Revista Ceres, Viçosa, MG, v. 57, n. 1, p. 112-117, jan./fev. 2010. DOI: 10.1590/S0034-737X2010000100018.
Abstract: Propagation of Passiflora setacea DC. is extremely difficult, therefore tissue culture techniques become a viable alternative. The objective of this study was to evaluate the in vitro germination and determine the best culture medium for micropropagation. This work was carried out in two stages; in the first, seeds were placed in % MS culture medium and 30 g L-1 of sucrose, distributed in tubes and supplemented with different AG3 concentrations. The pH of culture medium was adjusted for 5.8 before adding 6.0 mg L-1 of agar. After seed inoculation, the cultures were kept in a growth room under 35 µmol.m-2.s-1, 26±1ºC and photoperiod of 16 hours. Just after germination, the seedlings were transferred to tubes containing % MS, constituting a second experiment, in order to test culture media with different sucrose concentrations. The experiments were arranged in a complete randomized design, in a 3x3 factorial, with four repetitions and 15 seeds/plot (first experiment) and a 4x4 factorial, with four repetitions and 3 plants/plot (second experiment). The best results for micropropagation were obtanied in MSM medium with 28.51 and 28.74 g L-1 of sucrose. The highest germination speed index was obtained with 20 mg L-1 of AG3 combined with seed tip scarification.
URI: http://repositorio.ufla.br/jspui/handle/1/38059
Appears in Collections:DAG - Artigos publicados em periódicos

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