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metadata.artigo.dc.title: Colchicine and amiprophos-methyl (APM) in polyploidy induction in banana plant
metadata.artigo.dc.creator: Rodrigues, Filipe Almendagna
Soares, Joyce Dória Rodrigues
Santos, Roseneide Rocha dos
Pasqual, Moacir
Silva, Sebastião de Oliveira e
metadata.artigo.dc.subject: Musa acuminata
Flow cytometry
Tissue culture
metadata.artigo.dc.publisher: Academic Journals 12-Oct-2011
metadata.artigo.dc.identifier.citation: RODRIGUES, F. A. et al. Colchicine and amiprophos-methyl (APM) in polyploidy induction in banana plant. African Journal of Biotechnology, [S.l.], v. 10, n. 2, p. 13476-13481, Oct. 2011. DOI: 10.5897/AJB11.1661.
metadata.artigo.dc.description.abstract: The objective was to assess the colchicine and amiprophos-methyl (APM) concentration and exposure period in the chromosome duplication of breed banana plants diploids. Banana stem tips were used from the following genotypes: breed diploids (1304-04 [Malaccensis x Madang (Musa acuminata spp. banksii)] and 8694-15 [0337-02 (Calcutta x Galeo) x SH32-63]). Colchicine was used at concentrations of 0 (control treatment), 1.25, 2.5 and 5.0 mM, while APM was used at 0 (control treatment), 40 and 80 μM, in solution under agitation (20 rpm), for 24 and 48 h periods. With the use of APM, 66.67% tetraploid plants were obtained in the 1304-04 genotype using 40 μM for 24 h and 18.18% in 80 μM for 48 h, while in the 8694-15 genotype using 40 and 80 μM colchicine for 48 h, 27.27 and 21.43% tetraploid plants were observed, respectively. For colchicine, in the 1304-04 genotype, only the 1.25 mM treatment for 48 h presented 25% tetraploid plants and in the 8694-15 genotype, the 5.0 mM concentration for 48 h produced 50% tetraploid plants. APM for 24 h enabled the tetraploid plant of the 1304-04 genotype to be obtained, while colchicine for 48 h resulted in tetraploid plants in the 8694-15 genotype.
metadata.artigo.dc.language: en_US
Appears in Collections:DAG - Artigos publicados em periódicos

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