Please use this identifier to cite or link to this item:
Title: Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
Other Titles: Teor de isoflavonas e proteínas nos grãos de soja submetida ao alagamento em diferentes estádios de desenvolvimento
Keywords: Glycine max
Functional food
Alimento funcional
Issue Date: Dec-2011
Publisher: Universidade Federal de Santa Maria
Citation: FANTE, C. A. et al. Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development. Ciência Rural, Santa Maria, v. 41, n. 12, p. 2224-2229, Dec. 2011.
Abstract: The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.
Appears in Collections:DBI - Artigos publicados em periódicos

This item is licensed under a Creative Commons License Creative Commons