Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/38736
Título: Cryopreservation of embryonic axes of castor bean cultivar 'Al-Guarany 2002'
Palavras-chave: Castor bean seed
Ricinus communis
Oleaginous plant
Cryopreservation
Editor: International Society for Horticultural Science (ISHS)
Citação: NOGUEIRA, G. F. et al. Cryopreservation of embryonic axes of castor bean cultivar 'Al-Guarany 2002'. Acta Horticulturae, [S.l.], v. 908, p. 233-237, Sept. 2011. Proceedings of the I International Symposium on Cryopreservation in Horticultural Species, Leuven, Belgium, 2009. DOI: 10.17660/ActaHortic.2011.908.29.
Resumo: The castor bean (Ricinus communis) is an oleaginous plant with many applications in the society and economy. Castor bean seeds tolerate storage in seed banks for a short period compared to orthodox seeds, probably due to its high lipid content. This study aimed elaborate a cryopreservation protocol for castor bean embryonic axes. After seeds disinfection, embryonic axes of the cultivar ‘AL-Guarany 2002’ were isolated, dehydrated and inoculated directly on culture medium (control) or cryostored in liquid nitrogen at -196°C, during one hour, in the presence or absence of cryoprotectant solutions (PVS2 or modified PVS2) prior to inoculation on culture. It was obtained 100% of in vitro regeneration for all treatments. After acclimatization a surviving rate of 100% was obtained for embryonic axes cryostored only in liquid nitrogen. Axes stored in the presence of PVS2 presented a survival rate of 35%. The results obtained showed that the cryopreservation of castor bean embryonic axes in LN without cryoprotectants is a suitable technique to preserve the genetics resources of this species.
URI: https://www.actahort.org/books/908/908_29.htm
http://repositorio.ufla.br/jspui/handle/1/38736
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