Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/38798
metadata.artigo.dc.title: Partitioning of α-lactalbumin and β-lactoglobulin from cheese whey in aqueous two-phase systems containing poly (ethylene glycol) and sodium polyacrylat
metadata.artigo.dc.creator: Alcântara, Lizzy Ayra Pereira
Amaral, Isabella Vieira
Bonomo, Renata Cristina Ferreira
Silva, Luis Henrique Mendes da
Silva, Maria do Carmo Hespanhol da
Minim, Valéria Paula Rodrigues
Minim, Luis Antonio
metadata.artigo.dc.subject: Aqueous two-phase system
Partitioning
Whey protein
Optimization
Microcalorimetry
metadata.artigo.dc.publisher: Elsevier
metadata.artigo.dc.date.issued: Oct-2014
metadata.artigo.dc.identifier.citation: ALCÂNTARA, L. A. P. et al. Partitioning of α-lactalbumin and β-lactoglobulin from cheese whey in aqueous two-phase systems containing poly (ethylene glycol) and sodium polyacrylat. Food and Bioproducts Processing, [S.l.], v. 92, n. 4, p. 409-415, Oct. 2014.
metadata.artigo.dc.description.abstract: Partitioning behavior of the whey proteins α-lactalbumin (α-la) and β-lactoglobulin (β-lg) in aqueous two-phase systems prepared with poly ethylene glycol (PEG) and sodium polyacrilate (NaPA) was investigated as a function of pH and polymer concentrations. It was observed that α-la concentrated in the PEG phase while β-lg concentrated in the NaPA phase. Response surface methodology was applied to optimize protein partitioning and to achieve the best conditions for their fractionation. Thermodynamic analysis based on isothermal titration microcalorimetry indicated that the partitioning of α-la was accompanied by endothermic heat and was entropically driven, while β-lg partitioning was accompanied by exothermic heat and was enthalpically driven at low polymer concentrations and entropically driven at high polymer concentrations. Purification and yield parameters were determined using fresh whey and the results allowed for conclusion of the great applicability of this new system for α-la and β-lg fractionation.
metadata.artigo.dc.identifier.uri: https://www.sciencedirect.com/science/article/pii/S0960308513000977
http://repositorio.ufla.br/jspui/handle/1/38798
metadata.artigo.dc.language: en_US
Appears in Collections:DCA - Artigos publicados em periódicos

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