Organogenesis in three species of Annona (Annonaceae)

Abstract

This study aimed to evaluate, under in vitro conditions, the regenerative potential of leaves of three species of Annona (Annonaceae), A. squamosa, A. bahiensis and A. glabra, and hypocotyl and epicotyl segments of A. squamosa. In the first study, organogenesis was induced by inoculating the first leaves of three species of Annona in WPM medium supplemented with all combinations of concentrations of BAP (2.22, 4.44, 8.88 and 17.76 µM) and NAA (0.00, 1.34, 2.68 and 5.37 µM). The cotyledons and first leaves of A. squamosa were also cultivated in the same culture media. In the second study, hypocotyl and epicotyl segments of A. squamosa were inoculated in a medium supplemented with different concentrations of BAP (0.00, 2.22, 8.87, 17.74 and 35.48 µM). The results showed that organogenesis in Annona leaves is dependent on the type of explant, genotype and auxin-cytokinin balance used. In A. squamosa, the cotyledons had higher organogenic potential for micropropagation. Multiple shoots were regenerated from A. squamosa hypocotyls and epicotyls, but the regenerative potential of the hypocotyl was higher than that of the epicotyl.