Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/39000
Título: Influence of silver thiosulfate ethylene inhibitor on in vitro growth of Vasconcellea quercifolia shoots
Palavras-chave: Calli inhibition
In vitro rooting
Native species
Plant tissue culture
STS
Silver Thiosulfate
Editor: International Society for Horticultural Science (ISHS)
Citação: GALLO, C. M. et al. Influence of silver thiosulfate ethylene inhibitor on in vitro growth of Vasconcellea quercifolia shoots. Acta Horticulturae, [S.l.], v. 1083, p. 565-568, May 2015. Proceedings of the VIII International Symposium on In Vitro Culture and Horticultural Breeding, Coimbra, Portugal, 2013. DOI: 10.17660/ActaHortic.2015.1083.75.
Resumo: The Vasconcellea quercifolia is a native species, belonging to the Caricaceae family, which is resistant to Papaya ringspot virus that can be used in plant breeding programs for commercial papaya cultivars. The objective of this study was to investigate the influence of silver thiosulfate, an inhibitor of ethylene action, during the in vitro growth of Vasconcellea quercifolia shoots. Shoots around two inches long were inoculated on MS medium supplemented with 20 g L-1 sucrose, 10 ppm ascorbic acid, 0.1 μM BA and 1 μM IBA. The silver thiosulfate was filter-sterilized and different concentrations (0, 0.1, 1, 10 and 100 μM) were added to the autoclaved medium. Each treatment consisted of 10 replicates and the experiment was performed in duplicate. After 30 days of culture in a growth chamber, the following parameters were evaluated: (i) intensity of callus formation, (ii) number of green leaves, (iii) number of leaves showing chlorosis, (iv) explant length, (v) number and (vi) root length. The use of STS was statistically significant regarding callus formation at the shoots base, shoot length, number of green leaves, number of roots and root length. Higher callus formation was observed in the absence of silver thiosulfate. Longer explants (23 and 18 mm) were observed in the absence or presence of 0.1 μM silver thiosulfate, respectively. The highest silver thiosulfate concentration (100 µM) caused a reduction in the number of green leaves. The use of 0.1 and 1 µM STS showed better results for number of root (3.8 and 2.3) and root length (67 and 49 mm), respectively. The results show that ethylene interferes in the in vitro growth of Vasconcellea quercifolia shoots.
URI: https://www.actahort.org/books/1083/1083_75.htm
http://repositorio.ufla.br/jspui/handle/1/39000
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