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metadata.artigo.dc.title: Milt Cryopreservation for Rheophilic Fish Threatened by Extinction in the Rio Grande, Brazil
metadata.artigo.dc.creator: Souza, Estefânia de Souza
Paula, Daniella Aparecida de Jesus
Felizardo, Viviane de Oliveira
Murgas, Luis David Solis
Veras, Galileu Crovatto
Rosa, Priscila Vieira e
metadata.artigo.dc.subject: Fish - Reproduction
Brycon orbignyanus
Piaractus mesopotamicus
Prochilodus lineatus
Peixes - Reprodução
metadata.artigo.dc.publisher: Cryoletters Jan-2014
metadata.artigo.dc.identifier.citation: ANDRADE, E. de S. et al. Milt Cryopreservation for Rheophilic Fish Threatened by Extinction in the Rio Grande, Brazil. CryoLetters, [S. I.], v. 35, n. 1, p. 8-14, Jan. 2014.
metadata.artigo.dc.description.abstract: BACKGROUND: Specific protocols for milt cryopreservation have been established for some freshwater fish species. However, cryopreservation reduces sperm quality, giving unsatisfactory results in reproduction. OBJECTIVE: The objective of this work was to evaluate the effect of different cryoprotectants on the quality of Prochilodus lineatus, Brycon orbignyanus and Piaractus mesopotamicus milt after cryopreservation. METHODS: The milt was diluted in different cryoprotectant solutions containing 10% methanol, dimethyl sulfoxide, glycerol, propylene glycol or ethylene glycol combined with the Beltsville Thawing Solution extender (5%), then placed in the vapour of a liquid nitrogen (LN) storage tank for 24 h, after which they were immersed in LN. After rewarming, the rate (%) and duration (s) of milt motility and abnormal morphology were evaluated. RESULTS: All of cryoprotectant solutions tested used maintained the viability of P. lineatus and P. mesopotamicus milt. However, in P. lineatus, glycerol ensured a lower percentage of abnormal morphology. In case of P. mesopotamicus, all of the cryoprotectant solutions tested may be used in the cryopreservation process, with the exception of those containing glycerol. CONCLUSION: For B. orbignyanus, cryoprotectant solutions containing methanol and ethylene glycol are recommended for use in the cryopreservation process, although they reduced the quality of sperm post-rewarming.
metadata.artigo.dc.language: en
Appears in Collections:DZO - Artigos publicados em periódicos

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