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metadata.artigo.dc.title: Classification of the inoculant strain of cowpea UFLA03-84 and of other strains from soils of the Amazon region as Bradyrhizobium viridifuturi (symbiovar tropici)
metadata.artigo.dc.creator: Costa, Elaine Martins da
Carvalho, Teotonio Soares de
Guimarães, Amanda Azarias
Leão, Aniele Carolina Ribas
Cruz, Leonardo Magalhães
Baura, Valter Antonio de
Lebbe, Liesbeth
Willems, Anne
Moreira, Fatima Maria de Souza
metadata.artigo.dc.subject: Nitrogen fixing bacteria
Bactérias fixadoras de oxigênio
metadata.artigo.dc.publisher: Springer 2019
metadata.artigo.dc.identifier.citation: COSTA, E. M. da et al. Classification of the inoculant strain of cowpea UFLA03-84 and of other strains from soils of the Amazon region as Bradyrhizobium viridifuturi (symbiovar tropici). Brazilian Journal of Microbiology, [S. l.], v. 50, p. 335-345, 2019.
metadata.artigo.dc.description.abstract: Cowpea (Vigna unguiculata L.) is a legume species that considerably benefits from inoculation with nitrogen fixing bacteria of the genus Bradyrhizobium. One of the strains recommended for inoculation in cowpea in Brazil is UFLA03-84 (Bradyrhizobium sp.). The aim of our study was to define the taxonomic position of the UFLA03-84 strain and of two other strains of Bradyrhizobium (UFLA03-144 and INPA237B), all belonging to the same phylogenetic group and isolated from soils of the Brazilian Amazon. Multilocus sequence analysis (MLSA) of the housekeeping genes atpD, gyrB, recA, and rpoB grouped (with similarity higher than 99%) the three strains with Bradyrhizobium viridifuturi SEMIA 690T. The analyses of average nucleotide identity and digital DNA–DNA hybridization supported classification of the group as Bradyrhizobium viridifuturi. The three strains exhibited similar behavior in relation to the most of the phenotypic characteristics evaluated. However, some characteristics exhibited variation, indicating phenotypic diversity within the species. Phylogenetic analysis of the nodC and nifH genes showed that the three strains are members of the same symbiovar (tropici) that contains type strains of Bradyrhizobium species coming from tropical soils (SEMIA 690TB. viridifuturi, CNPSo 1112TB. tropiciagri, CNPSo 2833TB. embrapense, and B. brasilense UFLA03-321T).
metadata.artigo.dc.language: en_US
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