Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/40947
metadata.artigo.dc.title: Addition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar semen
metadata.artigo.dc.creator: Resende, Carla Oliveira
Betarelli, Rafael Pedroso
Rabelo, Stenia Severo
Chaves, Bruna Resende
Rodriguez-Gil, Joan Enric
Zangeronimo, Marcio Gilberto
metadata.artigo.dc.subject: Antioxidant
Boar spermatozoa
Cryopreservation
Lipid peroxidation
Semen
Thawed semen
Antioxidante
Suíno - Espermatozóides
Criopreservação
Peroxidação lipídica
Sêmen
Sêmen descongelado
metadata.artigo.dc.publisher: Elsevier B.V.
metadata.artigo.dc.date.issued: Sep-2019
metadata.artigo.dc.identifier.citation: RESENDE, C. O. et al. Addition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar sêmen. Animal Reproduction Science, [S.I.], v. 208, Sept. 2019. Não paginado.
metadata.artigo.dc.description.abstract: The objective of this study was to evaluate the effect of adding reduced glutathione (GSH) to a boar semen freezing extender supplemented with insulin-like growth factor I (IGF-I) or anti-IGF-I. Eight ejaculates from eight boars were extended to obtain insemination doses, which were supplemented with either recombinant human IGF-I (30 ng/mL) or anti-IGF-I (60 ng/mL) shortly after extension. After 24 h of liquid storage at 17 °C, the semen was frozen with or without GSH (5 mM) in the freezing extender for a total of six treatments. Osmotic resistance and acrosome integrity was greater in fresh semen (P <  0.05) soon after adding IGF-I or the anti-IGF-I antibody. After 24 h of cooling, the supplementation with these compounds resulted in an increased (P <  0.05) percentage of sperm with relatively greater mitochondrial activity and reduced the percentage of cells with relatively greater concentrations of superoxide. After thawing, there was a reduction (P <  0.05) in the percentage and fluorescence intensity of sperm with greater quantities of superoxide and peroxide only in samples treated with GSH + IGF-I and GSH + anti-IGF-I. The addition of GSH (alone or in combination with IGF-I or anti-IGF-I), however, reduced the percentage of sperm with an intact acrosome (P < 0.05). The same effect was not observed with IGF-I or anti-IGF-I alone. In conclusion, the addition of IGF-I or anti-IGF-I improved the quality of fresh or liquid-stored semen. Using GSH in the freezing extender improved the antioxidant potential of frozen semen only in combination with IGF-I or an anti-IGF-I antibody.
metadata.artigo.dc.identifier.uri: https://www.sciencedirect.com/science/article/abs/pii/S037843201930185X?via%3Dihub#!
http://repositorio.ufla.br/jspui/handle/1/40947
metadata.artigo.dc.language: en
Appears in Collections:DMV - Artigos publicados em periódicos

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