Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/42488
Título: Gene expression and genetic control to cold tolerance during maize seed germination
Palavras-chave: Zea mays
Seed vigor
Heterosis
Maternal effect
Milho - Semente
Semente - Vigor
Heterose
Genética - Efeito materno
Data do documento: Abr-2020
Editor: Springer Nature
Citação: SILVA NETA, I. C. et al. Gene expression and genetic control to cold tolerance during maize seed germination. BMC Plant Biology, [S. I.], v. 20, n. 188, 2020. DOI: https://doi.org/10.1186/s12870-020-02387-3.
Resumo: Background: The study of cold tolerance in maize seeds and seedlings through physiological quality assessments, as well as the genetic control associated with this trait, allows an early characterization of genotypes. Here we studied the genetic control for cold tolerance during the germination process in maize seeds and genes influenced by this stress. Results: Six maize lines were used, three classified as tolerant and three as susceptible to low germination temperature. A field was developed to produce the hybrid seeds, in a partial diallel scheme including the reciprocal crosses. For the expression analysis, seeds from two contrasting lines were used, as well as their hybrid combination and their reciprocal crosses, on dried and moistened seeds at 10 °C for 4 and 7 days. It was evaluated the catalase (CAT) and esterase (EST) enzymes, heat-resistant proteins and the genes Putative stearoyl-ACP desaturase (SAD), Ascorbate Peroxidase (APX), Superoxide Dismutase (SOD) and Mitogen Activated Protein Kinase (ZmMPK5). The estimated values ​​for heterosis, general and specific combining abilities and reciprocal maternal and non-maternal effects were carried out and it showed that there is heterosis for germination at low temperatures, also the non-additive genes were more important and there was a reciprocal effect. Conclusions: There is a greater expression of the CAT and EST enzymes in moistened seeds at seven days and there is less expression of heat-resistant proteins and the SAD gene at seven days of moistening. Also, there are variations in the expression of the APX, SOD and ZmMPK5 genes in dried and moistened seeds, as well as among the genotypes studied.
URI: http://repositorio.ufla.br/jspui/handle/1/42488
Aparece nas coleções:DAG - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
ARTIGO_Gene expression and genetic control to cold tolerance during maize seed germination.pdf1,06 MBAdobe PDFVisualizar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons