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metadata.artigo.dc.title: Gene expression and genetic control to cold tolerance during maize seed germination
metadata.artigo.dc.creator: Silva Neta, Izabel Costa
Von Pinho, Édila Vilela de Resende
Abreu, Viviane Maria de
Vilela, Danielle Rezende
Santos, Milena Christy
Santos, Heloisa Oliveira dos
Ferreira, Ricardo Augusto Diniz Cabral
Von Pinho, Renzo Garcia
Vasconcellos, Renato Coelho de Castro
metadata.artigo.dc.subject: Zea mays
Seed vigor
Maternal effect
Milho - Semente
Semente - Vigor
Genética - Efeito materno
metadata.artigo.dc.publisher: Springer Nature Apr-2020
metadata.artigo.dc.identifier.citation: SILVA NETA, I. C. et al. Gene expression and genetic control to cold tolerance during maize seed germination. BMC Plant Biology, [S. I.], v. 20, n. 188, 2020. DOI:
metadata.artigo.dc.description.abstract: Background: The study of cold tolerance in maize seeds and seedlings through physiological quality assessments, as well as the genetic control associated with this trait, allows an early characterization of genotypes. Here we studied the genetic control for cold tolerance during the germination process in maize seeds and genes influenced by this stress. Results: Six maize lines were used, three classified as tolerant and three as susceptible to low germination temperature. A field was developed to produce the hybrid seeds, in a partial diallel scheme including the reciprocal crosses. For the expression analysis, seeds from two contrasting lines were used, as well as their hybrid combination and their reciprocal crosses, on dried and moistened seeds at 10 °C for 4 and 7 days. It was evaluated the catalase (CAT) and esterase (EST) enzymes, heat-resistant proteins and the genes Putative stearoyl-ACP desaturase (SAD), Ascorbate Peroxidase (APX), Superoxide Dismutase (SOD) and Mitogen Activated Protein Kinase (ZmMPK5). The estimated values ​​for heterosis, general and specific combining abilities and reciprocal maternal and non-maternal effects were carried out and it showed that there is heterosis for germination at low temperatures, also the non-additive genes were more important and there was a reciprocal effect. Conclusions: There is a greater expression of the CAT and EST enzymes in moistened seeds at seven days and there is less expression of heat-resistant proteins and the SAD gene at seven days of moistening. Also, there are variations in the expression of the APX, SOD and ZmMPK5 genes in dried and moistened seeds, as well as among the genotypes studied.
metadata.artigo.dc.language: en
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