Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/42493
metadata.artigo.dc.title: qPCR-based detection of Colletotrichum truncatum in soybean seeds
metadata.artigo.dc.creator: Silva Júnior, Manoel Batista da
Resende, Mário Lúcio Vilela de
Pozza, Edson Ampélio
Botelho, Deila Magna Dos Santos
Cardoso, Acleide Maria Santos
Siqueira, Carolina da Silva
Machado, José da Cruz
Resende, Alexandre Ribeiro Maia de
Silveira, Gustavo Cesar Dias
Guimarães, Sarah da Silva Costa
metadata.artigo.dc.subject: Glycine max - Diseases
Molecular detection
Plant disease - Diagnostic
Specific primers
Soja - Sementes - Doenças
Antracnose
Detecção molecular
Doença de planta - Diagnóstico
Primers específicos
metadata.artigo.dc.publisher: Springer Nature
metadata.artigo.dc.date.issued: Jul-2020
metadata.artigo.dc.identifier.citation: SILVA JÚNIOR, M. B. et al. qPCR-based detection of Colletotrichum truncatum in soybean seeds. Tropical Plant Pathology, [S. I.], 2020. DOI: https://doi.org/10.1007/s40858-020-00380-7.
metadata.artigo.dc.description.abstract: Soybean seed infected with Colletotrichum truncatum is an important source of primary inoculum for anthracnose epidemics. Based on differences in the GAPDH gene sequences of Colletotrichum species, one pair of species-specific primers, CtruncF1/CtruncR1, was designed to accurately detect C. truncatum in soybean seed samples. The primers amplified only a single PCR band of 211 bp from C. truncatum. SYBR Green qPCR using these primers enabled the detection of DNA of the target fungus in inoculated soybean seeds and in naturally infested seeds. The sensitivity of the method was 0.000253 ng/μL of C. truncatum DNA template, with an efficiency of 1.78 and a Ct of 30.09. These species-specific primers may be useful for certification of soybean seeds aimed at avoiding introduction of the pathogen into soybean-producing regions.
metadata.artigo.dc.identifier.uri: https://doi.org/10.1007/s40858-020-00380-7
http://repositorio.ufla.br/jspui/handle/1/42493
metadata.artigo.dc.language: en
Appears in Collections:DFP - Artigos publicados em periódicos

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