Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/42684
Título : SARS-CoV-2 detection by direct rRT-PCR without RNA extraction
Autor: Merindol, Natacha
Pépin, Geneviève
Marchand, Caroline
Rheault, Marylène
Peterson, Christine
Poirier, André
Houle, Claudia
Germain, Hugo
Danylo, Alexis
Palavras-chave: COVID-19
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Coronavirus
Virus detection
Direct rRTPCR
RNA extraction
Publicador: Elsevier
Data da publicação: Jul-2020
Referência: MERINDOL, N. et al. SARS-CoV-2 detection by direct rRT-PCR without RNA extraction. Journal of Clinical Virology, [S.l.], v. 128, July 2020.
Abstract: Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona’s assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium.
URI: https://www.sciencedirect.com/science/article/pii/S1386653220301657
http://repositorio.ufla.br/jspui/handle/1/42684
Idioma: en_US
Aparece nas coleções:FCS - Artigos sobre Coronavirus Disease 2019 (COVID-19)

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