Please use this identifier to cite or link to this item:
metadata.artigo.dc.title: Δ-FeOOH as support for immobilization peroxidase: optimization via a chemometric approach
metadata.artigo.dc.creator: Tavares, Tássia Silva
Rocha, Eduardo Pereira da
Nogueira, Francisco Guilherme Esteves
Torres, Juliana Arriel
Silva, Maria Cristina
Kuca, Kamil
Ramalho, Teodorico de Castro
metadata.artigo.dc.subject: Horseradish peroxidase
Iron oxide hydroxide
metadata.artigo.dc.publisher: Multidisciplinary Digital Publishing Institute (MDPI) Jan-2020
metadata.artigo.dc.identifier.citation: TAVARES, T. S. et al. Δ-FeOOH as support for immobilization peroxidase: optimization via a chemometric approach. Molecules, Basel, v. 25, n. 2, 2020. DOI:10.3390/molecules25020259.
metadata.artigo.dc.description.abstract: Owing to their high surface area, stability, and functional groups on the surface, iron oxide hydroxide nanoparticles have attracted attention as enzymatic support. In this work, a chemometric approach was performed, aiming at the optimization of the horseradish peroxidase (HRP) immobilization process on Δ-FeOOH nanoparticles (NPs). The enzyme/NPs ratio (X1), pH (X2), temperature (X3), and time (X4) were the independent variables analyzed, and immobilized enzyme activity was the response variable (Y). The effects of the factors were studied using a factorial design at two levels (−1 and 1). The biocatalyst obtained was evaluated for the ferulic acid (FA) removal, a pollutant model. The materials were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The SEM images indicated changes in material morphology. The independent variables X1 (−0.57), X2 (0.71), and X4 (0.42) presented the significance effects estimate. The variable combinations resulted in two significance effects estimates, X1*X2 (−0.57) and X2*X4 (0.39). The immobilized HRP by optimized conditions (X1 = 1/63 (enzyme/NPs ratio, X2 = pH 8, X4 = 60 °C, and 30 min) showed high efficiency for FA oxidation (82%).
metadata.artigo.dc.language: en
Appears in Collections:DQI - Artigos publicados em periódicos

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.