Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/43593
Registro completo de metadados
Campo DCValorIdioma
dc.creatorFalasca, Francesca-
dc.creatorSciandra, Ilaria-
dc.creatorDi Carlo, Daniele-
dc.creatorGentile, Massimo-
dc.creatorDeales, Alberto-
dc.creatorAntonelli, Guido-
dc.creatorTurriziani, Ombretta-
dc.date.accessioned2020-10-29T18:02:45Z-
dc.date.available2020-10-29T18:02:45Z-
dc.date.issued2020-12-
dc.identifier.citationFALASCA, F. et al. Detection of SARS-COV N2 Gene: very low amounts of viral RNA or false positive? Journal of Clinical Virology, [S.l.], v. 133, Dec. 2020.pt_BR
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1386653220304029pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/43593-
dc.description.abstractBackground The detection of a low amount of viral RNA is crucial to identify a SARS-CoV-2 positive individual harboring a low level of virus, especially during the convalescent period. However, the detection of one gene at high Cycle threshold (Ct) has to be interpreted with caution. In this study we address this specific issue and report our real-life experience. Study design A total of 1639 nasopharyngeal swabs (NPS) were analyzed with Xpert® Xpress SARS-CoV-2. Positive samples showing high Ct values (Ct>35) were concentrated by centrifugation and re-tested with Cepheid or other methods (RealStar SARS-CoV2 RT-PCR, Altona Diagnostics; GeneFinder COVID-19 Plus RealAmp Kit, Elitech). Results 1599 (97.5%) negative samples, 36 (2.3%) positive samples and 4 (0.2%) presumptive positive samples were detected. In 17 out of 36 positive patients, very low viral RNA copies were suspected since positivity was detected at high Ct. We confirmed positivity for patients who showed both E and N genes detected and for patients with only N detected but with Ct <39. On the contrary, samples with only gene N detected with Ct values >39 were found negative. NPS taken 24 hours after the first collection confirmed the negativity of the 12 samples. Clinical data sustained these results since only 2 of these 12 patients showed COVID-19-like symptoms. Conclusions These data support our consideration that detection of the N2 gene at high Ct needs to be interpreted with caution, suggesting that collaboration between virologists and clinicians is important for better understanding of results.pt_BR
dc.languageen_USpt_BR
dc.publisherElsevierpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceJournal of Clinical Virologypt_BR
dc.subjectSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)pt_BR
dc.subjectCOVID-19 - Diagnosispt_BR
dc.subjectFalse positivept_BR
dc.subjectN2 genept_BR
dc.subjectCt valuept_BR
dc.titleDetection of SARS-COV N2 Gene: very low amounts of viral RNA or false positive?pt_BR
dc.typeArtigopt_BR
Aparece nas coleções:FCS - Artigos sobre Coronavirus Disease 2019 (COVID-19)

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.