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Campo DCValorIdioma
dc.creatorBastos, Sabrina Carvalho-
dc.creatorPimenta, Carlos José-
dc.creatorDias, Disney Ribeiro-
dc.creatorChalfoun, Sara Maria-
dc.creatorAngélico, Caroline Lima-
dc.creatorTavares, Lucas Silva-
dc.date.accessioned2014-10-27T14:05:20Z-
dc.date.available2014-10-27T14:05:20Z-
dc.date.issued2013-
dc.identifier.citationBASTOS, S. C. et al. Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean. World Journal of Agricultural Sciences, [S.l.], v. 9, n. 2, p. 167-172, 2013.pt_BR
dc.identifier.urihttp://www.idosi.org/wjas/wjas9(2)13/9.pdfpt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/4487-
dc.description.abstractTo examine tThe optimal conditions of cultivation for Cladosporium cladosporioides to produce and partially purify pectin methylesterase (PME) and polygalacturonase (PG) were examined under laboratory conditions . Activities of PME and PG were evaluated after 10, 15 and 20 days of incubation at 28°C in rice solid medium. Enzyme extraction was carried out by homogenization in buffer solutions at different pH (4, 5 and 6) followed by precipitation with ammonium sulfate [(NH4)2SO4] at 20, 40 and 60% saturation. After 10 days of incubation, the highest activities for PG (105.5 U/mL) and PME (1480 U/mL) in the crude extract was observed. Benzoate buffer (pH 4.0) was the best buffer for enzymes extraction. The final yield of 108.7% for PME and 10.6% for PG and a purification index of 14.2 and 1.4 for PME and PG, respectively, was obtained after saturation with 60%. The ideal conditions for obtained and pre-purification of PME and PG were 10 days of incubation, extraction with benzoate buffer pH 4.0 and precipitation with 60% of (NH4)2SO4. This is the first report on of pectinases pectinolytic enzymes produced by from C. cladosporioides and provides information about growth conditions for production and pre-purification of PME and PG.pt_BR
dc.languageenpt_BR
dc.publisherWorld Science Research Journals (WSRJ)pt_BR
dc.rightsrestritopt_BR
dc.sourceWorld Journal of Agricultural Sciences (Print)pt_BR
dc.subjectPectinolytic enzymespt_BR
dc.subjectFilamentous fungipt_BR
dc.subjectPectinpt_BR
dc.subjectCoffee fermentationpt_BR
dc.titlePectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee beanpt_BR
dc.typeArtigopt_BR
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