Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/48047
metadata.artigo.dc.title: Complete genome sequence of the biocontrol agent Serratia marcescens strain N4–5 uncovers an assembly artefact
metadata.artigo.dc.creator: Ferreira, Larissa Carvalho
Maul, Jude E.
Viana, Marcus Vinicius Canário
Sousa, Thiago Jesus de
Azevedo, Vasco Ariston de Carvalho
Roberts, Daniel P.
Souza, Jorge Teodoro de
metadata.artigo.dc.subject: Serratia marcescens
Artefacts
5S rRNA
Biological control
Complete genome
Plant-associated bacteria
RNA ribossômico 5S
Controle biológico
Sequenciamento completo do genoma
Bactérias associadas a plantas
metadata.artigo.dc.publisher: Springer Nature
metadata.artigo.dc.date.issued: Sep-2020
metadata.artigo.dc.identifier.citation: FERREIRA, L. C. et al. Complete genome sequence of the biocontrol agent Serratia marcescens strain N4–5 uncovers an assembly artefact. Brazilian Journal of Microbiology, [S. I.], v. 52, p. 245–250, Mar. 2021. DOI: https://doi.org/10.1007/s42770-020-00382-2.
metadata.artigo.dc.description.abstract: Serratia marcescens are gram-negative bacteria found in several environmental niches, including the plant rhizosphere and patients in hospitals. Here, we present the genome of Serratia marcescens strain N4–5 (=NRRL B-65519), which has a size of 5,074,473 bp (664-fold coverage) and contains 4840 protein coding genes, 21 RNA genes, and an average G + C content of 59.7%. N4–5 harbours a plasmid of 11,089 bp and 43.5% G + C content that encodes six unique CDS repeated 2.5× times totalling 13 CDS. Our genome assembly and manual curation uncovered the insertion of two extra copies of the 5S rRNA gene in the assembled sequence, which was confirmed by PCR and Sanger sequencing to be a misassembly. This artefact was subsequently removed from the final assembly. The occurrence of extra copies of the 5S rRNA gene was also observed in most complete genomes of Serratia spp. deposited in public databases in our comparative analysis. These elements, which also occur naturally, can easily be confused with true genetic variation. Efforts to discover and correct assembly artefacts should be made in order to generate genome sequences that represent the biological truth underlying the studied organism. We present the genome of N4–5 and discuss genes potentially involved in biological control activity against plant pathogens and also the possible mechanisms responsible for the artefact we observed in our initial assembly. This report raises awareness about the extra copies of the 5S rRNA gene in sequenced bacterial genomes as they may represent misassemblies and therefore should be verified experimentally.
metadata.artigo.dc.identifier.uri: https://doi.org/10.1007/s42770-020-00382-2
http://repositorio.ufla.br/jspui/handle/1/48047
metadata.artigo.dc.language: en
Appears in Collections:DFP - Artigos publicados em periódicos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.