Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/48830
Título: Development and characterization of electrochemical sensors for residual determination of tricaine (MS-222) and benzocaine in fish
Autores: Saczk, Adelir Aparecida
Oliveira, Juliano Elvis de
Felix, Fabiana da Silva
Brito, Ângela Dayana Barrera de
Baccaro, Alexandre Luiz Bonizio
Ferreira, Lucas Franco
Cardoso, Maria das Graças
Palavras-chave: Anestésicos - Análise residual
Tilápia-do-Nilo
Polímeros condutores
Planejamento fatorial fracionado
Anesthetics
Nile tilapia
Fractional factorial design
Conductive polymers
Data do documento: 13-Jan-2022
Editor: Universidade Federal de Lavras
Citação: LIMA, R. M. F. de. Development and characterization of electrochemical sensors for residual determination of tricaine (MS-222) and benzocaine in fish. 2021. 143 p. Tese (Doutorado em Agroquímica) - Universidade Federal de Lavras, Lavras, 2018.
Resumo: During the aquaculture management process, anesthetics are used in order to stagnant fish and facilitate production activities. This makes there a fish meat quality improvement, because reducing the stress to the animal; the final product has better meat texture, besides reducing the mortality rate during production. However, the misuse of these chemicals can leave residual traces in fish meat, generating concern about the consumer's food safety. In this sense, the objective in this work was to develop efficient electrochemical/electroanalytical methodologies for the application in the determination of residual anesthetics in fish fillets. In the first article, the study of electrosynthesis of an electrode based on a conductive organic polymer associated with carboxylic acid functionalized carbon nanotube (GCE/PANI-MWNT's) was carried out using the cyclic voltammetry technique, wherein the parameters were optimized using statistical technique of fractional factorial planning. In this work, it was possible to correlate electrochemical tests with two levels and six factors (26-1) and optimize the electrode preparation, characterized by FTIR and Raman analyze. In the second article, the electrode (GCE/PANI-MWTC's) was applied in the residual determination of tricaine in fish using the voltammetric DPV technique. For the electrode characterization, the EIS technique was used, demonstrating the relation of the potential scanning cycle and the polymer chain formation. The analytical method developed showed detection limits 2.08 x 10-8 mol L-1 and the quantification 4.00 x 10-8 mol L-1 with correlation coefficient 0.9905. The extraction method presented efficiency in the tricaine determination of fish filet with recovery 90% ± 4%. In addition, proposals were made of mechanisms interaction between polyaniline (PANI), carboxylic acid functionalized carbon nanotube (MWTC's) and tricaine anesthetic. In the third article, benzocaine and residual tricaine were determined in fish fillets using a printed carbon electrode using BIA methodology. It was possible to obtain more than 300 injections per hour with low detection limits 3.02 x 10-8 mol L-1 for benzocaine and 3.19 x 10-8 mol L-1 for tricaine, using only 80 mL of sample for each analysis, with high repeatability and good recovery rate of both anesthetics > 99%.
URI: http://repositorio.ufla.br/jspui/handle/1/48830
Aparece nas coleções:Agroquímica - Doutorado (Teses)



Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.