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dc.creatorSantos, Milena Christy-
dc.creatorVon Pinho, Édila Vilela de Resende-
dc.creatorSantos, Heloisa Oliveira dos-
dc.creatorVilela, Danielle Rezende-
dc.creatorSilva Neta, Izabel Costa-
dc.creatorAbreu, Viviane Maria de-
dc.creatorVasconcellos, Renato Coelho de Castro-
dc.date.accessioned2022-07-22T19:48:09Z-
dc.date.available2022-07-22T19:48:09Z-
dc.date.issued2021-
dc.identifier.citationSANTOS, M. C. et al. Enzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlings. Bioscience Journal, Uberlândia, v. 37, 2021.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/50696-
dc.description.abstractDrought stress is a major limiting factor for the development of maize, and the identification of the expression of genes related to this stress in seeds and seedlings can be an important tool to accelerate the selection process. The expression of genes related to tolerance to water deficit in seeds and in different tissues of maize seedlings were evaluated. Four tolerant genotypes (91-T, 32-T, 91x75-T, 32x75-T) and four non-tolerant genotypes (37-NT, 57-NT, 37x57-NT and 31x37-NT) were seeded in a substrate with 10% (stress) and 70% (control) water retention capacity. The expression of 4 enzymes were evaluated: catalase (CAT), peroxidase (PO), esterase (EST), and heat-resistant protein (HRP), as well as the relative expression of 6 genes: ZmLEA3, ZmPP2C, ZmCPK11, ZmDREB2A/2.1s, ZmDBP3 and ZmAN13 were evaluated in seed, shoots and roots of seedlings submitted or not to stress. There was variation in the expression of CAT, PO, SOD, EST and HRP enzymes among the evaluated genotypes and also in the different tissues evaluated. Higher expression of the CAT and PO was observed in the shoots. There was a greater expression of the EST in the genotypes non-tolerant to water deficit. HRP was expressed only in seeds. In the aerial part of maize seedlings, classified as tolerant, higher expression of genes ZmLEA3 and ZmCPK11 was observed. There was a higher expression of the ZmAN13 and ZmDREB2A/2.1S genes in roots developed under stress conditions and a higher expression of the ZmPP2C gene in seeds of line 91-T, which is classified as tolerant to drought stress.pt_BR
dc.languageen_USpt_BR
dc.publisherUniversidade Federal de Uberlândiapt_BR
dc.rightsAttribution 4.0 International*
dc.rightsacesso abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceBioscience Journalpt_BR
dc.subjectAbiotic stresspt_BR
dc.subjectProteomicpt_BR
dc.subjectRT-qPCRpt_BR
dc.subjectZea mayspt_BR
dc.titleEnzymatic activity and gene expression related to drought stress tolerance in maize seeds and seedlingspt_BR
dc.typeArtigopt_BR
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